TGIF1 Antibody

Western blot shows lysates of HCT‑116 human colorectal carcinoma cell line and A549 human lung carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human TGIF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7555) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for TGIF1 at approximately 32-34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

TGIF1 Antibody Summary

• Immunogen: E. coli-derived recombinant human TGIF1
  Lys245-Ser359
  Accession # Q15583
• Specificity: Detects human TGIF1 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: TGIF1
• Purity: Immunogen affinity purified
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TGIF1 Antibody

• 5'-TG-3' interacting factor
• homeobox protein TGIF
• homeobox protein TGIF1
• HPE4
• MGC39747
• TALE homeobox TG-interacting factor
• TGFB-induced factor (TALE family homeobox)
• TGFB-induced factor homeobox 1
• TGIF1
• TGIFMGC5066,5'-TG-3'-interacting factor 1
• transforming growth factor-beta-induced factor

Background

TGIF1 (5'-TG[TCA]-3' Interacting Protein 1; also Transforming growth factor beta-induced factor and HPE4) is a 43 kDa (predicted) member of the TALE/TGIF homeobox family of proteins. It is widely expressed, and serves as a transcriptional corepressor for SMAD2-induced gene activation. Evidence suggests that it either complexes with c-jun and cPML, or interacts with HDAC in the nucleus to perform this function. It also binds to RXR response elements, blocking RXR-mediated gene activation. Such interactions are of considerable importance during fetal and neonatal development. Human TGIF1 is 401 amino acids (aa) in length. It contains a DNA-binding homeobox domain (aa 164-226) and two consecutive repressor domains (aa 267-401). There are multiple splice variants. One is very well characterized, is 33-35 kDa in size, and shows a four aa substitution for aa 1-133. This is most active when phosphorylated, which adds approximately 2-4 kDa to its SDS-PAGE MW. Two other splice forms contain a 19 aa substitution for aa 1-134, and utilize an alternative start site at Met150, respectively. Over aa 245-359, human TGIF1 shares 81% aa identity with mouse TGIF1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TGIF1 Antibody (AF7555)(1)

Publication using AF7555

• Shahinian H, Loessner D, Biniossek M, Kizhakkedathu J, Clements J, Magdolen V, Schilling O Secretome and degradome profiling shows that Kallikrein-related peptidases 4, 5, 6, and 7 induce TGFbeta-1 signaling in ovarian cancer cells. Mol Oncol, 2014;8(1):68-82. 2014 [PMID: 24120346] (Western Blot, Human)

Bioinformatics

• Gene Symbol: TGIF1
• Uniprot:
  • Q15583(Human)