TGF beta induced factor 2 Antibody

Western Blot: TGF beta induced factor 2 Antibody [NB600-803] - (0.3ug/ml) staining of A431 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Po, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

TGF beta induced factor 2 Antibody Summary

• Immunogen: Peptide with sequence C-EMELQKQQDP corresponding to internal region according to NP_068581.1.
• Predicted Species: Mouse (100%), Rat (100%), Porcine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TGIF2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.3 - 10 ug/ml
• Application Notes: WB: Approx. 22 kDa band observed in A431, A549 and HEK293 lysates (calculated MW of 25.9 kDa band according to NP_068581.1).
• Control:

  A-431 Whole Cell Lysate
  TGF beta induced factor 2 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TGF beta induced factor 2 Antibody

• homeobox protein TGIF2
• TGF(beta)-induced transcription factor 2
• TGF-beta-induced transcription factor 2,5'-TG-3'-interacting factor 2,5'-TG-3' interacting factor 2
• TGFB-induced factor 2 (TALE family homeobox)
• TGFB-induced factor 2
• TGFB-induced factor homeobox 2
• transcription growth factor-beta-induced factor 2

Background

The protein encoded by this gene is a DNA-binding homeobox protein and a transcriptional repressor. The encoded protein appears to repress transcription by recruiting histone deacetylases to TGF beta-responsive genes. This gene is amplified and overexpressed in some ovarian cancers, and mutations in this gene can cause holoprosencephaly.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TGF beta induced factor 2 Antibody (NB600-803)(1)

Publication using NB600-803

• Melhuish TA, Gallo CM, Wotton D. TGIF2 interacts with histone deacetylase 1 and represses transcription. J Biol Chem 2001-08-24 [PMID: 11427533]

Bioinformatics

• Gene Symbol: TGIF2
• Entrez:
  • 60436(Human)
  • 228839(Mouse)
  • 681373(Rat)
• Uniprot:
  • NP_068581.1(Human)