TGF-beta 2 Antibody

Western Blot: TGF-beta 2 Antibody [NBP1-45892] - Inhibition of miR-10b suppresses angiogenesis by U87-2M1 glioma cells. Downregulation of expression of pro-angiogenic proteins in U87-2M1 cells by miR-10b decoy as demonstrated in western blotting analysis. Numbers indicate relative protein expression in miR-10b decoy-treated U87-2M1 cells compared with control decoy-transduced U87-2M1 cells after normalization to beta-actin levels. Blots shown are representative of biological triplicates. Image collected and cropped by CiteAb from the following publication (//www.nature.com/doifinder/10.1038/cddis.2012.134), licensed under a CC-BY license.

Western Blot: TGF-beta 2 Antibody [NBP1-45892] - Analysis of TGF-beta 2 using Jurkat cell lysate (Lane 1,2), Mouse 3T3 cell lysate (Lane 3) and Rat kidney tissue lysate (Lane 4).

Product Discontinued

TGF-beta 2 Antibody Summary

• Immunogen: Synthetic peptide surrounding amino acid 366 of human TGF-beta 2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TGFB2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 - 4 ug/mL

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 0.5% BSA, 30% Glycerol
• Preservative: 0.01% Thimerosal
• Concentration: 0.5 mg/ml
• Purity: Affinity purified

Alternate Names for TGF-beta 2 Antibody

• BSC-1 cell growth inhibitor
• cetermin
• Glioblastoma-derived T-cell suppressor factor
• G-TSF
• MGC116892
• polyergin
• TGFB2
• TGFbeta 2
• TGF-beta 2
• TGF-beta2
• TGF-beta-2
• transforming growth factor beta-2
• transforming growth factor, beta 2

Background

Transforming Growth Factor (TGF) betas mediate many cell to cell interactions that occur during embryonic development. Three TGF betas have been identified in mammals. TGF beta 1, TGF beta 2 and TGF beta 3 are each synthesized as precursor proteins that are very similar in that each is cleaved to yield a 112 amino acid polypeptide that remains associated with the latent portion of the molecule. The TGF beta polypeptides are multifunctional; capable of influencing cell proliferation, differentiation, and other functions in a wide range of cell types. Transformed, as well as nonneoplastic tissues, release transforming growth factors; and essentially all mammalian cells possess a specific TGF receptor. The multi modal nature of TGF beta is seen in its ability to stimulate or inhibit cellular proliferation. In general, cells of mesenchymal origin appear to be stimulated by TGF beta whereas cells of epithelial or neuroectodermal origin are inhibited by the peptide. TGF beta 1, TGF beta 2, and TGF beta 1.2 appear to be equivalent in biological activity, although there does appear to be differences in binding to certain types of receptors. TGF beta 2 is produced by many cell types and has been found in the highest concentration in porcine platelets and mammalian bone. Latent TGF beta 2 is the prominent isoform found in body fluids such as amniotic fluid, breast milk, and the aqueous and vitreous humor of the eye.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for TGF-beta 2 Antibody (NBP1-45892)(1)

Publication using NBP1-45892

• Lin J, Teo S, Lam DH, Jeyaseelan K, Wang S. MicroRNA-10b pleiotropically regulates invasion, angiogenicity and apoptosis of tumor cells resembling mesenchymal subtype of glioblastoma multiforme Cell Death Dis 2012-10-04 [PMID: 23034333] (WB, Human)

Bioinformatics

• Gene Symbol: TGFB2
• Entrez:
  • 7042(Human)
• Uniprot:
  • P61812()