TGF-beta 2 Antibody [Unconjugated]

Images

 
Simple Western lane view shows lysates of human heart tissue and human breast cancer tissue, loaded at 0.2 mg/mL. A specific band was detected for TGF‑ beta 2 at approximately 63 kDa (as indicated) using 2.5 ...read more
Western blot shows lysates of human heart tissue and human breast cancer tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-TGF‑ beta 2 Polyclonal Antibody (Catalog # AB-112-NA) followed by ...read more
Porcine TGF‑ beta 2 (Catalog # 102-B2) inhibits Recombinant Mouse IL‑4 (Catalog # 404-ML) induced proliferation in the HT‑2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant ...read more

Product Details

Summary
Reactivity MultiSpecies Glossary
Applications WB, Simple Western, Neut
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

TGF-beta 2 Antibody [Unconjugated] Summary

Immunogen
Porcine platelet-derived TGF-beta 2
Specificity
Detects TGF-beta 2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody is highly specific for TGF-beta 2 and
TGF‑ beta 1.2 but will cross-react with other TGF-beta variants with at least 100-fold lower sensitivity. It will also neutralize the biological activity of TGF-beta 1.2, at a 50 fold higher IgG concentration.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TGFB2
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.25 ug/mL
  • Simple Western 2.5 ug/mL
  • Neutralization 0.3-1.5 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 1 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TGF-beta 2 Antibody [Unconjugated]

  • BSC-1 cell growth inhibitor
  • cetermin
  • Glioblastoma-derived T-cell suppressor factor
  • G-TSF
  • MGC116892
  • polyergin
  • TGFB2
  • TGFbeta 2
  • TGF-beta 2
  • TGF-beta2
  • TGF-beta-2
  • transforming growth factor beta-2
  • transforming growth factor, beta 2

Background

TGF-beta 2 (transforming growth factor beta 2) is one of three closely related mammalian members of the large TGF-beta  superfamily that share a characteristic cysteine knot structure (1-7). TGF-beta 1, -2 and -3 are highly pleiotropic cytokines proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition (1-4). Each TGF-beta isoform has some non-redundant functions; for TGF-beta 2, mice with targeted deletion show defects in development of cardiac, lung, craniofacial, limb, eye, ear and urogenital systems (2). Human TGF-beta 2 cDNA encodes a 414 amino acid (aa) precursor that contains a 19 aa signal peptide and a 395 aa proprotein (8). A furin-like convertase processes the proprotein to generate an N-terminal 232 aa latency-associated peptide (LAP) and a C-terminal 112 aa mature TGF-  beta 2 (8, 9). Disulfide-linked homodimers of LAP and TGF-beta 2 remain non-covalently associated after secretion, forming the small latent TGF-beta 1 complex (8-10). Covalent linkage of LAP to one of three latent TGF-beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix (9, 10). TGF-beta is activated from latency by pathways that include actions of the protease plasmin, matrix metalloproteases, thrombospondin 1 and a subset of integrins (10). Mature human TGF-beta 2 shows 100% aa identity with porcine, canine, equine and bovine TGF-beta 2, and 97% aa identity with mouse and rat TGF-beta 2. It demonstrates cross-species activity (1). TGF-beta 2 signaling begins with binding to a complex of the accessory receptor betaglycan (also known as TGF-beta  RIII) and a type II ser/thr kinase receptor termed TGF-beta  RII. This receptor then phosphorylates and activates another ser/thr kinase receptor, TGF-beta  RI (also called activin receptor-like kinase (ALK) -5), or alternatively, ALK-1. The whole complex phosphorylates and activates Smad proteins that regulate transcription (3, 11, 12). Use of other signaling pathways that are Smad-independent allows for disparate actions observed in response to TGF-beta in different contexts (11).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol TGFB2
Entrez