TGF-beta 2 Antibody (771213)

Recombinant Mouse TGF‑ beta 2 (Catalog # 7346‑B2) inhibits Recombinant Mouse IL-4 (Catalog # 404-ML) induced proliferation in the HT‑2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse IL-4 (7.5 ng/mL) activity elicited by Recombinant Mouse TGF-beta 2 (5 ng/mL) is neutralized (green line) by increasing concen­trations of Rat Anti-Mouse TGF‑ beta 2 Monoclonal Antibody (Catalog # MAB7346). The ND50 is typically 0.15-0.75 ug/mL.

• Reactivity: Mu
• Applications: B/N
• Clone: 771213
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

TGF-beta 2 Antibody (771213) Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant mouse TGF‑ beta 2
  Ala303-Ser414
  Accession # P27090
• Specificity: Detects mouse TGF‑ beta 2 in direct ELISAs. In direct ELISAs, approximately 50% cross-reactivity with recombinant human (rh) TGF-beta 2 and rhTGF-beta 3 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: TGFB2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 0.15-0.75 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TGF-beta 2 Antibody (771213)

• BSC-1 cell growth inhibitor
• cetermin
• Glioblastoma-derived T-cell suppressor factor
• G-TSF
• MGC116892
• polyergin
• TGFB2
• TGFbeta 2
• TGF-beta 2
• TGF-beta2
• TGF-beta-2
• transforming growth factor beta-2
• transforming growth factor, beta 2

Background

TGF-beta 2 (transforming growth factor beta 2) is one of three closely related mammalian members of the large TGF-beta  superfamily that share a characteristic cysteine knot structure. TGF-beta 1, -2 and -3 are highly pleiotropic cytokines proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition. Each TGF-beta isoform has some non-redundant functions; for TGF-beta 2, mice with targeted deletion show defects in development of cardiac, lung, craniofacial, limb, eye, ear and urogenital systems. Mouse TGF-beta 2 cDNA encodes a 414 amino acid (aa) precursor that contains a 19 aa signal peptide and a 395 aa proprotein. A furin-like convertase processes the proprotein to generate an N-terminal 283 aa latency-associated peptide (LAP) and a C-terminal 112 aa mature TGF-  beta 2. Disulfide-linked homodimers of LAP and TGF-beta 2 remain non-covalently associated after secretion, forming the small latent TGF-beta 2 complex. Covalent linkage of LAP to one of three latent TGF-beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix. TGF-beta is activated from latency by pathways that include actions of the protease plasmin, matrix metalloproteases, thrombospondin 1 and a subset of integrins. Mature mouse TGF-beta 2 shares 100% aa identity with rat TGF-beta 2, and 97% aa identity with human, porcine, canine, equine and bovine TGF-beta 2. It demonstrates cross-species activity. In most cells, TGF-beta 2 signaling begins with binding to a complex of the accessory receptor betaglycan (also known as TGF-beta  RIII) and a type II ser/thr kinase receptor termed TGF-beta  RII, which then phosphorylates and activates another ser/thr kinase receptor, TGF-beta  RI (also called activin receptor-like kinase (ALK) -5), or alternatively, ALK-1. The whole complex phosphorylates and activates Smad proteins that regulate transcription. In bone -related cells, however, TGF-beta 2 also signals through TGF-beta RIIB (a splice variant of TGF-beta RII), independently of TGF-beta RIII. Use of other signaling pathways that are Smad-independent allows for disparate actions observed in response to TGF-beta in different contexts.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TGF-beta 2 Antibody (MAB7346)(5)

Publications using MAB7346

• P Bernasconi, N Carboni, G Ricci, G Siciliano, L Politano, L Maggi, T Mongini, L Vercelli, C Rodolico, E Biagini, G Boriani, L Ruggiero, L Santoro, E Schena, S Prencipe, C Evangelist, E Pegoraro, L Morandi, M Columbaro, C Lanzuolo, P Sabatelli, P Cavalcante, C Cappellett, G Bonne, A Muchir, G Lattanzi Elevated TGF β2 serum levels in Emery-Dreifuss muscular dystrophy: implications for myocyte and tenocyte differentiation and fibrogenic processes Nucleus, 2018-01-01;0(0):1-24. 2018-01-01 [PMID: 29693488] (Neutralization, Mouse)
• XW Chen, TJ Yu, J Zhang, Y Li, HL Chen, GF Yang, W Yu, YZ Liu, XX Liu, CF Duan, HL Tang, M Qiu, CL Wang, H Zheng, J Yue, AM Guo, J Yang CYP4A in tumor-associated macrophages promotes pre-metastatic niche formation and metastasis Oncogene, 2017-05-08;0(0):. 2017-05-08 [PMID: 28481877] (Functional Assay, Mouse)
• Contreras-Ruiz L, Masli S Immunomodulatory cross-talk between conjunctival goblet cells and dendritic cells. PLoS ONE, 2015-03-20;10(3):e0120284. 2015-03-20 [PMID: 25793763] (Neutralization, Mouse)
• Luna-Gomes T, Filardy A, Rocha J, Decote-Ricardo D, LaRocque-de-Freitas I, Morrot A, Bozza P, Castro-Faria-Neto H, DosReis G, Nunes M, Freire-de-Lima C Neutrophils increase or reduce parasite burden in Trypanosoma cruzi-infected macrophages, depending on host strain: role of neutrophil elastase. PLoS ONE, 2014-03-05;9(3):e90582. 2014-03-05 [PMID: 24599360] (Bioassay, Mouse)
• Di Bernardini E, Campagnolo P, Margariti A, Zampetaki A, Karamariti E, Hu Y, Xu Q Endothelial lineage differentiation from induced pluripotent stem cells is regulated by microRNA-21 and transforming growth factor beta2 (TGF-beta2) pathways. J Biol Chem, 2013-12-19;289(6):3383-93. 2013-12-19 [PMID: 24356956] (Neutralization, Mouse)

Bioinformatics

• Gene Symbol: TGFB2
• Uniprot:
  • P27090()