Recombinant Human TGF-beta 1 Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE, HPLC
Concentration
Lyoph

Order Details

Recombinant Human TGF-beta 1 Protein Summary

Description
A recombinant protein corresponding to two identical 112 amino acids of Human TGF-beta 1
Preparation
Method
>98% SDS-PAGE
Details of Functionality
The ED50 as determined by the ability of TGF-B1 to inhibit the mouse IL-4-dependent proliferation of mouse HT-2 cells is <0.05 ng/ml, corresponding to a specific activity of >2 x 107 units/mg.
Source
CHO
Protein/Peptide Type
Biologically Active Protein
Gene
TGFB1
Purity
>95%, by SDS-PAGE
Endotoxin Note
<0.1 ng/ug

Applications/Dilutions

Theoretical MW
25.0 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from Sodium Citrate, pH3.5 buffer
Concentration
Lyoph
Purity
>95%, by SDS-PAGE
Reconstitution Instructions
Reconstitution in H2O to a concentration of 50 ug/ml (e.g., 5 ug/100 l) will yield a solution of 1 mM citrate buffer (pH 3.5). The solution can then be stored at 4C for 1 month. It is recommended that further dilutions be made in PBS containing 2 mg/ml albumin and stored at -20C.

Alternate Names for Recombinant Human TGF-beta 1 Protein

  • CEDLAP
  • DPD1
  • latency-associated peptide
  • TGFB
  • TGFB1
  • TGF-beta 1 protein
  • TGFbeta 1
  • TGF-beta 1
  • TGFbeta
  • TGF-beta-1
  • transforming growth factor beta-1
  • transforming growth factor, beta 1

Background

TGF-Beta 1 (Transforming Growth Factor-Beta 1) is a member of the superfamily of homologous, disulfide-linked, homodimeric proteins that regulate the proliferation and differentiation of normal and transformed cells. Human TGF-Beta 1 is a 25.0 kDa protein containing two identical 112 amino acid polypeptide chains linked by a single disulfide bond.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.

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Blogs on TGF-beta 1.

TGF-beta 1 - a versatile signaling molecule with roles in development and disease
The transforming growth factor-beta (TGF-beta) family consists of a wide variety of signaling proteins with roles in development. TGF-beta signaling controls growth, differentiation, and immune responses and is often misregulated in cancer. TGF-beta...  Read full blog post.

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Bioinformatics

Gene Symbol TGFB1
Entrez