Human TGF-beta 1 ELISA Kit (Colorimetric)

ELISA: TGF-beta 1 ELISA Kit [NBP1-91252] - Standard curve

Product Discontinued

Human TGF-beta 1 ELISA Kit (Colorimetric) Summary

• Description: The human TGF-beta1 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human TGF-beta1.
• Specificity: The assay detects both natural and recombinant human TGF beta 1. The cross reactivity of TGF beta 2 and TGF beta 3, and of TNF beta, IL- 8, IL-6, IL-2, TNF alpha, IL-1 beta, IL-4, IFN gamma, IL-12p70, IL-5 and IL-10 was evaluated by spiking these proteins at physiologically relevant concentrations into serum. There was no cross reactivity detected.
• Standard Curve Range: 31.3 - 2,000 pg/mL
• Sensitivity: 9.0 pg/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 4.90%
• Intra-Assay: CV% < 3.20%
• Spike Recovery: Please see protocol for more specific Spike Recovery details.
• Sample Volume: 40 uL (pretreated)
• Kit Type: ELISA Kit (Colorimetric)
• Gene: TGFB1

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human TGF-beta 1 ELISA Kit (Colorimetric)

• CEDLAP
• DPD1
• latency-associated peptide
• TGF beta
• TGF beta1
• TGFB
• TGFB1
• TGF-beta 1 protein
• TGFbeta 1
• TGF-beta 1
• TGFbeta
• TGF-beta-1
• transforming growth factor beta-1
• transforming growth factor, beta 1

Background

TGF-beta plays a critical role in cellular growth, development, differentiation, proliferation, extracellular matrix (ECM) synthesis and degradation, control of mesenchymal-epithelial interactions during embryogenesis, immune modulation, apoptosis, cell cycle progression, angiogenesis, adhesion and migration and leukocyte chemotaxis. It has both tumor suppressive and tumor promoting activities and is highly regulated at all levels. TGF-beta1 is the key mediator in the pathophysiology of tissue repair and human fibrogenesis: balance between production and degradation of type I collagen, and fibrosis and scarring in organ and tissue. TGF-beta1 promotes Treg cell differentiation resulting in IL-10/TGF-beta1 production and Th1 cell and Th2 cell suppression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for TGF-beta 1 ELISA Kit (NBP1-91252)(2)

Publications using NBP1-91252

• Kamal NM, Shams NS. The impact of tobacco smoking and electronic cigarette vaping on salivary biomarkers. A comparative study The Saudi Dental Journal 2022-07-01 [PMID: 35814841]
• Xiao JF, Kua LF, Ding LW et al. KDM6A Depletion in Breast Epithelial Cells Leads to Reduced Sensitivity to Anticancer Agents and Increased TGF beta Activity Molecular cancer research : MCR 2022-01-12 [PMID: 35022315]

FAQs for TGF-beta 1 ELISA Kit (NBP1-91252). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: TGFB1