Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS (pH 7.2)
0.05% Sodium Azide
Immunogen affinity purified
Western Blot 1:500-1:1000
Western blot (WB) analysis of TGF Beta1 (Y369) pAb in extracts from HepG2 cells.Immunohistochemistry (IHC) analyzes of TGFBeta1 (Y369) pAb in paraffin-embedded human breast carcinoma tissue. Use in chromatin immunoprecipitation reported in scientific literature (PMID 24311432) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
44 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using NB100-91995 in the following applications:
Transforming growth factor betas (TGFbetas) were originally discovered due to their ability to promote anchorage-independent growth of rat NRK fibroblasts in the presence of TGFalpha. It is now realized that TGFbetas mediate many cell-cell interactions that occur during embryonic development. Three TGFbetas have been identified in mammals. TGFbeta1, TGFbeta2 and TGFbeta3 are each synthesized as precursor proteins that are very similar in that each is cleaved to yield a 112 amino acid polypeptide that remains associated with the latent portion of the molecules. Biologically active TGFbeta requires dimerization of the monomers (usually homodimers) and release of the latent peptide portion. Overall, the mature region of the TGFbeta3 protein has approximately 80% identity to the mature region of both TGFbeta1 and TGFbeta2. However, the NH2 terminals or precursor regions of their molecules share only 27% sequence identity.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for TGF-beta 1 Antibody (NB100-91995). (Showing 1 - 1 of 1 FAQs).
Could you tell me if this antibody can be used for TGF beta detection on an ELISA test for example? I saw that it is affinity purified but also Tyr 369 modified. What is the impact of the tyr 369 modification?
This antibody has been tested in an ELISA to detect TGF beta, so it appears to me an ideal antibody for your research. The Tyr 369 modification means that the antibody will bind specifically within the region of the 369th Amino Acid, in this case Tyrosine. It would have no negative effect on the ELISA test. We will guarantee this antibody 100% for use in ELISA to detect TGF beta 1.