TGF-beta 1 Antibody

Images

 
Western Blot: TGF-beta 1 Antibody [NB100-91995] - Western blot (WB) analysis of TGF beta-1 (Y369) pAb in extracts from HepG2 cells.
Immunohistochemistry-Paraffin: TGF-beta 1 Antibody [NB100-91995] - Paraffin-embedded human breast carcinoma tissue.
Western Blot: TGF-beta 1 Antibody [NB100-91995] - Lane1:Hela cell lysate. Lane2:SP2/0 cell lysate. Lane3:H9C2 cell lysate. Lane4:MCF-7 cell lysate.

Product Details

Summary
Product Discontinued
View other related TGF-beta 1 Primary Antibodies

Order Details


    • Catalog Number
      NB100-91995
    • Availability
      Product Discontinued

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TGF-beta 1 Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 341-390 of Human TGFbeta1.
Clonality
Polyclonal
Host
Rabbit
Gene
TGFB1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
Application Notes
Western blot (WB) analysis of TGF Beta1 (Y369) pAb in extracts from HepG2 cells.Immunohistochemistry (IHC) analyzes of TGFBeta1 (Y369) pAb in paraffin-embedded human breast carcinoma tissue. Use in chromatin immunoprecipitation reported in scientific literature (PMID 24311432) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
44 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NB100-91995 in the following applications:

  • 1 publication
  • IHC
    1 publication
  • 1 publication
  • IP
    1 publication
  • WB
    2 publications

Alternate Names for TGF-beta 1 Antibody

  • CEDLAP
  • DPD1
  • latency-associated peptide
  • TGFB
  • TGFB1
  • TGF-beta 1 protein
  • TGFbeta 1
  • TGF-beta 1
  • TGFbeta
  • TGF-beta-1
  • transforming growth factor beta-1
  • transforming growth factor, beta 1

Background

Transforming growth factor betas (TGFbetas) were originally discovered due to their ability to promote anchorage-independent growth of rat NRK fibroblasts in the presence of TGFalpha. It is now realized that TGFbetas mediate many cell-cell interactions that occur during embryonic development. Three TGFbetas have been identified in mammals. TGFbeta1, TGFbeta2 and TGFbeta3 are each synthesized as precursor proteins that are very similar in that each is cleaved to yield a 112 amino acid polypeptide that remains associated with the latent portion of the molecules. Biologically active TGFbeta requires dimerization of the monomers (usually homodimers) and release of the latent peptide portion. Overall, the mature region of the TGFbeta3 protein has approximately 80% identity to the mature region of both TGFbeta1 and TGFbeta2. However, the NH2 terminals or precursor regions of their molecules share only 27% sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TGF-beta 1 Antibody (NB100-91995)(6)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-P, IP, WB.


Filter By Application
ICC/IF
(1)
IHC
(1)
IHC-P
(1)
IP
(1)
WB
(2)
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Mouse
(2)
Rat
(2)
All Species

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TGF-beta 1 Antibody (NB100-91995). (Showing 1 - 1 of 1 FAQs).

  1. Could you tell me if this antibody can be used for TGF beta detection on an ELISA test for example? I saw that it is affinity purified but also Tyr 369 modified. What is the impact of the tyr 369 modification?
    • This antibody has been tested in an ELISA to detect TGF beta, so it appears to me an ideal antibody for your research. The Tyr 369 modification means that the antibody will bind specifically within the region of the 369th Amino Acid, in this case Tyrosine. It would have no negative effect on the ELISA test. We will guarantee this antibody 100% for use in ELISA to detect TGF beta 1.

Secondary Antibodies

 

Isotype Controls

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Research Areas for TGF-beta 1 Antibody (NB100-91995)

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Blogs on TGF-beta 1.

TGF-beta 1 - a versatile signaling molecule with roles in development and disease
The transforming growth factor-beta (TGF-beta) family consists of a wide variety of signaling proteins with roles in development. TGF-beta signaling controls growth, differentiation, and immune responses and is often misregulated in cancer. TGF-beta...  Read full blog post.

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Bioinformatics

Gene Symbol TGFB1
Entrez
Uniprot