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NTS2/NTSR2 Antibody

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Western Blot: Neurotensin Receptor 2 Antibody [NB100-56472] - Analysis of neurotensin receptor 2 in the 1) absence and 2) presence of immunizing peptide in mouse brain lysate using this antibody at 2 ug/ml.
Western Blot: Neurotensin Receptor 2 Antibody [NB100-56472] - Analysis of neurotensin receptor 2 in the 1) absence and 2) presence of immunizing peptide in mouse brain lysate using this antibody at 2 ug/ml.
Immunohistochemistry-Paraffin: Neurotensin Receptor 2 Antibody [NB100-56472] - Analysis of human brain, cerebellum using this antibody at 10 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, BvSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB100-56472AF350
Alexa Fluor 405
NB100-56472AF405
Alexa Fluor 488
NB100-56472AF488
Alexa Fluor 532
NB100-56472AF532
Alexa Fluor 594
NB100-56472AF594
Alexa Fluor 647
NB100-56472AF647
Alexa Fluor 700
NB100-56472AF700
Alexa Fluor 750
NB100-56472AF750
Biotin
NB100-56472B
DyLight 350
NB100-56472UV
DyLight 405
NB100-56472V
DyLight 488
NB100-56472G
DyLight 550
NB100-56472R
DyLight 594
NB100-56472DL594
DyLight 650
NB100-56472C
DyLight 680
NB100-56472FR
DyLight 755
NB100-56472IR
FITC
NB100-56472F
HRP
NB100-56472H
Janelia Fluor 549
NB100-56472JF549
Janelia Fluor 646
NB100-56472JF646
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NTS2/NTSR2 Antibody Summary

Immunogen
A portion of amino acids 150-200 of human Neurotensin receptor 2 was used as the immunogen.
Predicted Species
Bovine (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NTSR2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot 0.2-2 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 10 ug/ml
Control
Human Brain Whole Tissue Lysate (Adult Whole Normal)
Publications
Read Publication using
NB100-56472 in the following applications:

  • WB
    1 publication

Reactivity Notes

Reacts with Human and Mouse. Predicted Reactivity: Rat, Dog, Bovine and Mouse

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS containing 0.2% gelatin
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for NTS2/NTSR2 Antibody

  • neurotensin receptor 2
  • neurotensin receptor type 2
  • neurotensin receptor, type 2
  • NT-R-2
  • NTR2Levocabastine-sensitive neurotensin receptor
  • NTS2
  • NTSR2

Background

Official Gene Symbol: NTSR2 Gen Bank Accession Number: NP_036476 Gene ID: 23620 (Human) Gene Map Locus: 2p25.1 (Human) NTR2 is a member of GPCR family. It contains a short N-terminal sequence, 7 transmembrane domains and a short cytoplasmic tail. It is a low affinity levocabastine-sensitive receptor for Neurotensin. Activation of NT2R by non-peptide agonists suggests that the receptor can couple to phospholipase C, phospholipase A2 and MAPK. Unlike NTR1, NTR2 is insensitive to GTP and has low sensitivity of sodium ions. Northern Blot analysis detected its expression in adult brain regions including the olfactory system, cerebral and crebellar cortices, hippocampus and hypothalamic nuclei. Its expression has also been found in low levels in kidney, uterus, heart and lung.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunohistochemistry-Paraffin: Neurotensin Antibody [NBP2-33903] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.
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Sortilin was detected in perfusion fixed frozen sections of mouse brain (rostral ventral medulla) using Goat Anti-Mouse Sortilin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2934) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
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Simple Western lane view shows lysates of mouse brain (cortex) tissue, loaded at 0.2 mg/mL. A specific band was detected for Sortilin at approximately 122 kDa (as indicated) using 10 µg/mL of Goat Anti-Mouse Sortilin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2934) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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Publications for Neurotensin Receptor 2 Antibody (NB100-56472)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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Product General Protocols

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Video Protocols

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FAQs for Neurotensin Receptor 2 Antibody (NB100-56472) (0)

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Control Lysate(s)

Human Brain Whole Tissue Lysate (Adult Whole Normal)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-56472AF350
Alexa Fluor 405 NB100-56472AF405
Alexa Fluor 488 NB100-56472AF488
Alexa Fluor 532 NB100-56472AF532
Alexa Fluor 594 NB100-56472AF594
Alexa Fluor 647 NB100-56472AF647
Alexa Fluor 700 NB100-56472AF700
Alexa Fluor 750 NB100-56472AF750
Biotin NB100-56472B
DyLight 350 NB100-56472UV
DyLight 405 NB100-56472V
DyLight 488 NB100-56472G
DyLight 550 NB100-56472R
DyLight 594 NB100-56472DL594
DyLight 650 NB100-56472C
DyLight 680 NB100-56472FR
DyLight 755 NB100-56472IR
FITC NB100-56472F
HRP NB100-56472H
Janelia Fluor 549 NB100-56472JF549
Janelia Fluor 646 NB100-56472JF646
Learn about Custom Antibody Labeling

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Bioinformatics Tool for Neurotensin Receptor 2 Antibody (NB100-56472)

Discover related pathways, diseases and genes to Neurotensin Receptor 2 Antibody (NB100-56472). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Bioinformatics

Gene Symbol NTSR2
Entrez