TFIIE beta Antibody

Western Blot: TFIIE beta Antibody [NBP2-20610] - Sample (30 ug of whole cell lysate) A: Jurkat nucleus 10% SDS PAGE gel, diluted at 1:5000.

Chromatin Immunoprecipitation: TFIIE beta Antibody [NBP2-20610] - Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either control rabbit IgG or anti-TFIIE beta antibody. The precipitated DNA was detected by PCR with primer set targeting to GAPDH promoter or MyoD.

Immunoprecipitation: TFIIE beta Antibody [NBP2-20610] - IP Sample: Jurkat whole cell lysate/extract A : 30 ug whole cell lysate/extract of TFIIE beta protein expressing Jurkat cells B : Control with 2.5 ug of pre-immune rabbit IgG C : Immunoprecipitation of TFIIE beta by 2.5 ug of TFIIE beta antibody 12% SDS-PAGE The immunoprecipitated TFIIE beta protein was detected by TFIIE beta antibody diluted at 1:1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.

• Reactivity: Hu, RM
• Applications: WB, IP, ChIP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

TFIIE beta Antibody Summary

• Description: Novus Biologicals Rabbit TFIIE beta Antibody (NBP2-20610) is a polyclonal antibody validated for use in WB, IP and ChIP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human TFIIE beta. The exact sequence is proprietary.
• Localization: Nucleus
• Predicted Species: Rhesus Macaque (99%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GTF2E2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP) Assay dependent
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:1000-1:10000
• Theoretical MW: 33 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Bovine (87%), Mouse (86%), Rat (86%), Pig (87%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for TFIIE beta Antibody

• FE
• General transcription factor IIE subunit 2
• general transcription factor IIE, polypeptide 2 (beta subunit, 34kD)
• general transcription factor IIE, polypeptide 2, beta 34kDa
• TF2E2TFIIE-beta
• TFIIE-B
• transcription initiation factor IIE subunit beta

Background

Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase andDNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: GTF2E2
• Uniprot:
  • P29084(Human)