TFIIE-alpha Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: TFIIE-alpha Antibody [NBP3-35521] - Immunofluorescence analysis of U-2 OS cells using TFIIE-alpha Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Western Blot: TFIIE-alpha Antibody [NBP3-35521] - Western blot analysis of various lysates using TFIIE-alpha Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5S.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

TFIIE-alpha Antibody - BSA Free Summary

• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 230-439 of human TFIIE-alpha (NP_005504.2).
  
  Sequence:
  LAGGHHREAWATKGPSYEDLYTQNVVINMDDQEDLHRASLEGKSAKERPIWLRESTVQGAYGSEDMKEGGIDMDAFQEREEGHAGPDDNEEVMRALLIHEKKTSSAMAGSVGAAAPVTAANGSDSESETSESDDDSPPRPAAVAVHKREEDEEEDDEFEEVADDPIVMVAGRPFSYSEVSQRPELVAQMTPEEKEAYIAMGQRMFEDLFE
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GTF2E1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Western Blot 1:200 - 1:2000
• Theoretical MW: 49 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for TFIIE-alpha Antibody - BSA Free

• FE
• General transcription factor IIE 56 kDa subunit
• general transcription factor IIE subunit 1
• general transcription factor IIE, polypeptide 1 (alpha subunit, 56kD)
• general transcription factor IIE, polypeptide 1, alpha 56kDa
• TF2E1
• TFIIE-A
• TFIIE-alpha
• Transcription initiation factor IIE subunit alpha

Background

Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase andDNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GTF2E1