TFIIE-alpha Antibody - BSA Free

Images

 
Immunohistochemistry-Paraffin: TFIIE-alpha Antibody [NBP1-46195] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- GTF2E1/TFIIE-alpha used at a dilution of 1:1,000 (1ug/ml). ...read more
Immunoprecipitation: TFIIE-alpha Antibody [NBP1-46195] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-GTF2E1/TFIIE-alpha antibody used for IP at 3 mcg/mg ...read more

Product Details

Summary
Reactivity Hu, Bv, Pm-OrSpecies Glossary
Applications WB, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

TFIIE-alpha Antibody - BSA Free Summary

Description
Novus Biologicals Rabbit TFIIE-alpha Antibody - BSA Free (NBP1-46195) is a polyclonal antibody validated for use in IHC and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
Immunogen
The immunogen for this product maps to a region between residue 100 and 150 of human General Transcription Factor IIE, polypeptide 1, alpha, using the numbering given in entry NP_005504.2 (GeneID 2960).
Predicted Species
Bovine (100%), Orangutan (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GTF2E1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:500-1:2000
  • Immunohistochemistry-Paraffin 1:500-1:2000
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot Negative
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TFIIE-alpha Antibody - BSA Free

  • FE
  • General transcription factor IIE 56 kDa subunit
  • general transcription factor IIE subunit 1
  • general transcription factor IIE, polypeptide 1 (alpha subunit, 56kD)
  • general transcription factor IIE, polypeptide 1, alpha 56kDa
  • TF2E1
  • TFIIE-A
  • TFIIE-alpha
  • Transcription initiation factor IIE subunit alpha

Background

Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase andDNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GTF2E1