TFIIE-alpha Antibody (JE66-41)

Western Blot: TFIIE-alpha Antibody (JE66-41) [NBP3-33006] - Western blot analysis of TFIIE-alpha on different lysates with Rabbit anti-TFIIE-alpha antibody (NBP3-33006) at 1/1,000 dilution. Lane 1: K-562 cell lysate (15 ug/Lane) Lane 2: Jurkat cell lysate (15 ug/Lane) Lane 3: Daudi cell lysate (15 ug/Lane) Lane 4: HeLa cell lysate (15 ug/Lane) Lane 5: A431 cell lysate (15 ug/Lane) Lane 6: A549 cell lysate (15 ug/Lane) Lane 7: HepG2 cell lysate (15 ug/Lane) Lane 8: HEK-293 cell lysate (15 ug/Lane) Lane 9: NIH/3T3 cell lysate (15 ug/Lane) Lane 10: MCF7 cell lysate (15 ug/Lane) Lane 11: Mouse testis tissue lysate (30 ug/Lane) Lane 12: Rat testis tissue lysate (30 ug/Lane) Predicted band size: 49.5 kDa Observed band size: 60 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33006) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: JE66-41
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

TFIIE-alpha Antibody (JE66-41) Summary

• Description: Novus Biologicals Rabbit TFIIE-alpha Antibody (JE66-41) (NBP3-33006) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Synthetic peptide within Human TFIIE-alpha aa 390-439 / 439. (Uniprot: P29083)
• Localization: Nucleus.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: GTF2E1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Theoretical MW: 49.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for TFIIE-alpha Antibody (JE66-41)

• FE
• General transcription factor IIE 56 kDa subunit
• general transcription factor IIE subunit 1
• general transcription factor IIE, polypeptide 1 (alpha subunit, 56kD)
• general transcription factor IIE, polypeptide 1, alpha 56kDa
• TF2E1
• TFIIE-A
• TFIIE-alpha
• Transcription initiation factor IIE subunit alpha

Background

Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase andDNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GTF2E1