TFF3 Antibody [Biotin]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Biotin
• Concentration: LYOPH

TFF3 Antibody [Biotin] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human TFF3
  Ala21-Phe80
  Accession # Q07654
• Specificity: Detects human TFF3 in Western blots. In this format, less than 1% cross-reactivity with recombinant human (rh) TFF1 and rhTFF2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: TFF3
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TFF3 Antibody [Biotin]

• HITF, human intestinal trefoil factor10Intestinal trefoil factor
• ITF
• TFF3
• TFI
• trefoil factor 3 (intestinal)

Background

TFF3 (Trefoil factor 3; also hP1.B and intestinal trefoil factor) is a 7 kDa member of the trefoil factor family of protease‑resistant peptides. It is expressed by multiple epithelial cell types, acts in an autocrine or paracrine manner and contributes to epithelial cell migration and survival. Mature human TFF3 is 59 amino acids (aa) in length (aa 22 ‑ 80). It contains one P (or trefoil) domain that is characterized by the presence of three cysteine bond‑mediated loops (aa 30 ‑ 72). Human TFF3 exists as both a monomer and disulfide‑linked dimer. There are three potential isoform variants. One shows an alternate start site at Met8, a second shows a 50 aa N‑terminal extension and a third shows a 109 aa substitution for aa 29 ‑ 80. Over aa 21 ‑ 80, human TFF3 shares 77%, 68% and 72% aa identity with mouse, canine and rat TFF3, respectively

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TFF3 Antibody (BAF4407)(1)

Publication using BAF4407

• Schulze U, Hampel U, Sel S, Contreras-Ruiz L, Schicht M, Dieckow J, Diebold Y, Paulsen F Trefoil factor family peptide 3 (TFF3) is upregulated under experimental conditions similar to dry eye disease and supports corneal wound healing effects in vitro. Invest Ophthalmol Vis Sci, 2014-05-08;55(5):3037-42. 2014-05-08 [PMID: 24713479] (ELISA Development (Detection), Human)

Bioinformatics

• Gene Symbol: TFF3
• Uniprot:
  • Q07654()