TFF3 Antibody [Alexa Fluor® 750]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 750

TFF3 Antibody [Alexa Fluor® 750] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human TFF3
  Ala21-Phe80
  Accession # Q07654
• Specificity: Detects human Trefoil Factor 3 (TFF3) in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) TFF1 and rhTFF2 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TFF3 Antibody [Alexa Fluor® 750]

• HITF, human intestinal trefoil factor10Intestinal trefoil factor
• ITF
• TFF3
• TFI
• trefoil factor 3 (intestinal)

Background

TFF3 (Trefoil factor 3; also hP1.B and intestinal trefoil factor) is a 7 kDa member of the trefoil factor family of protease-resistant peptides. It is expressed by multiple epithelial cell types, acts in an autocrine or paracrine manner and contributes to epithelial cell migration and survival. Mature human TFF3 is 59 amino acids (aa) in length (aa 22-80). It contains one P (or trefoil) domain that is characterized by the presence of three cysteine bond-mediated loops (aa 30-72). Human TFF3 exists as both a monomer and disulfide-linked dimer. There are three potential isoform variants. One shows an alternate start site at Met8, a second shows a 50 aa N-terminal extension and a third shows a 109 aa substitution for aa 29-80. Over aa 21-80, human TFF3 shares 77%, 68%, and 72% aa identity with mouse, canine, and rat TFF3, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q07654()