TET1 Antibody (1462)

Images

 
Western Blot: TET1 Antibody (1462) [NBP2-15135] - A. 30 ug 293T whole cell lysate/extract B. 30 ug whole cell lysate/extract of DDDDK-human TET1-transfected 293T cells 5 % SDS-PAGE TET1 antibody dilution: 1:500
Immunocytochemistry/ Immunofluorescence: TET1 Antibody (GT1462) [NBP2-15135] - detects TET1 protein at nucleus by immunofluorescent analysis. Sample: TET1-transfected (right) or untransfected (left) 293T cells were ...read more
Immunohistochemistry-Paraffin: TET1 Antibody (1462) [NBP2-15135] - Detects TET1 protein at nucleus on HeLa xenograft by immunohistochemical analysis. Sample: Paraffin- Embedded HeLa xenograft. Dilution: 1:100
Western Blot: TET1 Antibody (GT1462) [NBP2-15135] - A. 50 ug whole cell lysate/extract from 293T cells transfected with scramble siRNA. B. 50 ug whole cell lysate/extract from TET1-knockdowned 293T cells.
Western Blot: TET1 Antibody (1462) [NBP2-15135] - NT2D1 whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with TET1 antibody [1462] diluted at 1:500.
Immunoprecipitation: TET1 Antibody (1462) [NBP2-15135] - Immunoprecipitation of TET1 protein from NT2D1 whole cell extracts using 5 ug of TET1 antibody [1462]. Western blot analysis was performed using TET1 antibody ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ChIP, ICC/IF, IHC, IHC-P, IP
Clone
1462
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

TET1 Antibody (1462) Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human TET1. The exact sequence is proprietary.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
TET1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Chromatin Immunoprecipitation 1:10 - 1:500
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
Theoretical MW
235 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for TET1 Antibody (1462)

  • bA119F7.1
  • CXXC finger 6
  • CXXC6
  • CXXC6FLJ10839
  • FLJ41442
  • KIAA1676CXXC-type zinc finger protein 6
  • LCX
  • LCXEC 1.14.11.n2
  • Leukemia-associated protein with a CXXC domain
  • methylcytosine dioxygenase TET1
  • Ten-eleven translocation 1 gene protein
  • ten-eleven translocation-1
  • tet oncogene 1

Background

Dioxygenase that specifically binds methylcytosine (5mC), a minor base in mammalian DNA found in repetitive DNA elements that is crucial for retrotransposon silencing and mammalian development. Catalyzes the conversion of methylcytosine (5mC) to 5-hydroxymethylcytosine (hmC). The clear function of 5-hydroxymethylcytosine (hmC) is still unclear but it may influence chromatin structure and recruit specific factors or may constitute an intermediate component in cytosine demethylation. 5-hydroxymethylcytosine (hmC) is present in ES cells and is enriched in the brain, especially in Purkinje neurons. May play a role in the fetal development of heart, lung and brain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for TET1 Antibody (NBP2-15135) (0)

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol TET1