GRF2 Antibody (OTI2F5)

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Western Blot: GRF2 Antibody (2F5) [NBP2-45515] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: ...read more
Immunohistochemistry: GRF2 Antibody (2F5) [NBP2-45515] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: GRF2 Antibody (2F5) [NBP2-45515] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: GRF2 Antibody (2F5) [NBP2-45515] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: GRF2 Antibody (2F5) [NBP2-45515] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: GRF2 Antibody (2F5) [NBP2-45515] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: GRF2 Antibody (2F5) [NBP2-45515] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: GRF2 Antibody (2F5) [NBP2-45515] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunofluorescence: GRF2 Antibody (2F5) [NBP2-45515] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY GRF2.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, MkSpecies Glossary
Applications WB, IHC, ICC/IF
Clone
OTI2F5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

GRF2 Antibody (OTI2F5) Summary

Immunogen
Full length human recombinant protein of human RAPGEF1(NP_941372) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
RAPGEF1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:400 - 1:4000
  • Immunohistochemistry 1:150
  • Immunofluorescence 1:100
Theoretical MW
122.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for GRF2 Antibody (OTI2F5)

  • C3GGRF2guanine nucleotide-releasing factor 2 (specific for crk proto-oncogene)
  • CRK SH3-binding GNRP
  • DKFZp781P1719
  • Guanine nucleotide-releasing factor 2
  • Protein C3G
  • Rap guanine nucleotide exchange factor (GEF) 1
  • rap guanine nucleotide exchange factor 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-70846AF350
Alexa Fluor 405 NBP2-70846AF405
Alexa Fluor 488 NBP2-70846AF488
Alexa Fluor 532 NBP2-70846AF532
Alexa Fluor 594 NBP2-70846AF594
Alexa Fluor 647 NBP2-70846AF647
Alexa Fluor 700 NBP2-70846AF700
Alexa Fluor 750 NBP2-70846AF750
Biotin NBP2-70846B
DyLight 350 NBP2-70846UV
DyLight 405 NBP2-70846V
DyLight 488 NBP2-70846G
DyLight 550 NBP2-70846R
DyLight 594 NBP2-70846DL594
DyLight 650 NBP2-70846C
DyLight 680 NBP2-70846FR
DyLight 755 NBP2-70846IR
FITC NBP2-70846F
HRP NBP2-70846H
Janelia Fluor 549 NBP2-70846JF549
Janelia Fluor 646 NBP2-70846JF646

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Bioinformatics

Gene Symbol RAPGEF1