TCF7L2 Antibody (CL11547) - Azide and BSA Free

Staining of human pancreas shows no nuclear positivity in exocrine glandular cells and islets of Langhorne's.

Staining of human lymph node shows very weak nuclear positivity in non-germinal center cells.

Staining of human rectum shows moderate nuclear positivity in glandular cells.

Staining of mouse thalamus shows strong nuclear positivity in neuronal cells.

Analysis in human cell line CAPAN-2.

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clone: CL11547
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

TCF7L2 Antibody (CL11547) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse TCF7L2 Antibody (CL11547) - Azide and BSA Free (NBP3-21197) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a synthetic peptide of Q9NQB0, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TCF7L2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:200- 1:500
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for TCF7L2 Antibody (CL11547) - Azide and BSA Free

• HMG box transcription factor 4
• hTCF-4
• T-cell factor 4
• T-cell factor-4 variant A
• T-cell factor-4 variant B
• T-cell factor-4 variant C
• T-cell factor-4 variant D
• T-cell factor-4 variant E
• T-cell factor-4 variant H
• T-cell factor-4 variant I
• T-cell factor-4 variant J
• T-cell factor-4 variant K
• T-cell factor-4 variant L
• T-cell factor-4 variant M
• T-cell factor-4 variant X2
• T-cell-specific transcription factor 4
• TCF-4T-cell factor-4 variant F
• TCF4T-cell factor-4 variant G
• transcription factor 7-like 2 (T-cell specific, HMG-box)
• transcription factor 7-like 2

Background

TCF7L2, or TCF4, is a high mobility group (HMG) box-containing transcription factor that plays a key role in the Wnt signaling pathway. TCF7L2 has been implicated in glucose homeostasis through the regulation of pro-glucagon gene expression, which encodes glucagon-like peptide 1 (GLP-1). Genetic variants of TCF7L2 have been linked to type 2 diabetes, and it has specifically been shown to have an important role in the regulation of both 946;-cell survival and function (4). Several splicing isoforms for TCF7L2 have been shown to be differentially expressed in tissues during cancer progression (3), and gene targeting studies indicate that TCF7L2 is required to maintain the crypt stem cells of the small intestine (1, 2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TCF7L2