TCF7L1/TCF3 Antibody (622782) [Alexa Fluor® 594]

• Reactivity: Hu
• Applications: IHC
• Clone: 622782
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 594

TCF7L1/TCF3 Antibody (622782) [Alexa Fluor® 594] Summary

• Immunogen: E.coli-derived recombinant human TCF7L1
  Lys429-Ser581
  Accession # Q9HCS4
• Specificity: Detects human TCF7L1 in direct ELISAs. In direct ELISAs, 5-10% cross-reactivity with recombinant human (rh) TCF-4 (aa 102-516) and rhTCF-12 is observed. No cross-reactivity with rhTCF7L1 (aa 151-320), rhTCF7L2, rhTCF-1, -2, -3, -4 (aa 241-365), rhLEF-1, o
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TCF7L1/TCF3 Antibody (622782) [Alexa Fluor® 594]

• HMG box transcription factor 3
• TCF3
• TCF-3
• TCF7L1
• transcription factor 7-like 1 (T-cell specific, HMG-box)
• transcription factor 7-like 1

Background

Transcription factor 7-like 1 (TCF7L1; also HMG box transcription factor 3, TCF-3) is a 63 kDa member of the TCF/LEF family of proteins.  Human TCF7L1 is 588 amino acids (aa) in length and contains one HMG box DNA-binding domain (aa 346-414).  Human TCF7L1 shares 95% aa sequence identity with mouse TCF7L1.  TCF7L1 is expressed in hair follicles and skin keratinocytes and at lower levels in stomach epithelium.  Functionally, it participates in the Wnt signaling pathway.  TCF7L1 binds to DNA and acts as a repressor in the absence of CTNNB1 and as an activator in its presence.  TCF7L1 is also necessary for the terminal differentiation of epidermal cells, the formation of keratohyalin granules, and the development of the barrier function of the epidermis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.