TCF7L1/TCF3 Antibody (622782)

TCF7L1 was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Human TCF7L1 Monoclonal Antibody (Catalog # MAB61161) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: IHC
• Clone: 622782
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

TCF7L1/TCF3 Antibody (622782) Summary

• Immunogen: E.coli-derived recombinant human TCF7L1
  Lys429-Ser581
  Accession # Q9HCS4
• Specificity: Detects human TCF7L1 in direct ELISAs. In direct ELISAs, 5-10% cross-reactivity with recombinant human (rh) TCF-4 (aa 102-516) and rhTCF-12 is observed. No cross-reactivity with rhTCF7L1 (aa 151-320), rhTCF7L2, rhTCF-1, -2, -3, -4 (aa 241-365), rhLEF-1, or recombinant mouse TCF-1 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: TCF7L1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TCF7L1/TCF3 Antibody (622782)

• HMG box transcription factor 3
• TCF3
• TCF-3
• TCF7L1
• transcription factor 7-like 1 (T-cell specific, HMG-box)
• transcription factor 7-like 1

Background

Transcription factor 7-like 1 (TCF7L1; also HMG box transcription factor 3, TCF-3) is a 63 kDa member of the TCF/LEF family of proteins.  Human TCF7L1 is 588 amino acids (aa) in length and contains one HMG box DNA-binding domain (aa 346-414).  Human TCF7L1 shares 95% aa sequence identity with mouse TCF7L1.  TCF7L1 is expressed in hair follicles and skin keratinocytes and at lower levels in stomach epithelium.  Functionally, it participates in the Wnt signaling pathway.  TCF7L1 binds to DNA and acts as a repressor in the absence of CTNNB1 and as an activator in its presence.  TCF7L1 is also necessary for the terminal differentiation of epidermal cells, the formation of keratohyalin granules, and the development of the barrier function of the epidermis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TCF7L1/TCF3 Antibody (MAB61161)(1)

Publication using MAB61161

• J Shan, J Shen, M Wu, H Zhou, J Feng, C Yao, Z Yang, Q Ma, Y Luo, Y Wang, C Qian Tcf7l1 acts as a suppressor for the self-renewal of liver cancer stem cells and is regulated by IGF/MEK/ERK signaling independent ofβ-catenin Stem Cells, 2019-08-26;0(0):. 2019-08-26 [PMID: 31322782] (IHC, Human)

Bioinformatics

• Gene Symbol: TCF7L1
• Uniprot:
  • Q9HCS4(Human)