TCF7/TCF1 Antibody [Alexa Fluor® 532]

• Reactivity: Hu
• Applications: WB, IHC, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 532

TCF7/TCF1 Antibody [Alexa Fluor® 532] Summary

• Immunogen: E. coli-derived recombinant human TCF7/TCF1
  Met116-His233
  Accession # P36402
• Specificity: Detects human TCF7/TCF1 in Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TCF7/TCF1 Antibody [Alexa Fluor® 532]

• FLJ36364
• MGC47735
• T-cell factor 1
• T-cell specific
• TCF1
• TCF7
• TCF-7
• transcription factor 7 (T-cell specific, HMG-box)

Background

TCF7 (Transcription factor 7; also T cell factor 1/TCF1) is a 25‑50 kDa member of the lymphoid enhancer binding factor family of proteins with 16 isoforms. It is expressed in thymocytes and mature T cells, and serves multiple purposes. In resting cells, TCF family members are transcriptional repressors, and are 25‑32 kDa in size. Following activation, large TCF7 isoforms predominate (42‑50 kDa), and serve a transcriptional activator function. Human TCF7 is 384 amino acids (aa) in length. This is likely an activating isoform that contains a beta -catenin binding domain (aa 1‑59), a DNA-binding HMG-box (aa 269‑337), and an NLS (aa 344‑348). The use of an alternate start site at Met116 seems to characterize repressor isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.