TCF7/TCF1 Antibody [Unconjugated]

Western blot shows lysates of Jurkat human acute T cell leukemia cell line and MOLT-4 human acute lymphoblastic leukemia cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human TCF7/TCF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5596) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for TCF7/TCF1 at approximately 60, 40, and 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

TCF7/TCF1 was detected in immersion fixed HCT-116 human colorectal carcinoma cell line using Human TCF7/TCF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5596) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

TCF7/TCF1 was detected in immersion fixed paraffin-embedded sections of human thymus using Goat Anti-Human TCF7/TCF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5596) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

TCF7/TCF1 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human TCF7/TCF1
  Met116-His233
  Accession # P36402
• Specificity: Detects human TCF7/TCF1 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TCF7
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 3-15 ug/mL
  • Western Blot 0.2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TCF7/TCF1 Antibody [Unconjugated]

• FLJ36364
• MGC47735
• T-cell factor 1
• T-cell specific
• TCF1
• TCF7
• TCF-7
• transcription factor 7 (T-cell specific, HMG-box)

Background

TCF7 (Transcription factor 7; also T cell factor 1/TCF1) is a 25‑50 kDa member of the lymphoid enhancer binding factor family of proteins with 16 isoforms. It is expressed in thymocytes and mature T cells, and serves multiple purposes. In resting cells, TCF family members are transcriptional repressors, and are 25‑32 kDa in size. Following activation, large TCF7 isoforms predominate (42‑50 kDa), and serve a transcriptional activator function. Human TCF7 is 384 amino acids (aa) in length. This is likely an activating isoform that contains a beta -catenin binding domain (aa 1‑59), a DNA-binding HMG-box (aa 269‑337), and an NLS (aa 344‑348). The use of an alternate start site at Met116 seems to characterize repressor isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TCF7/TCF1 Antibody (AF5596)(1)

Publication using AF5596

• Junbin Qian, Siel Olbrecht, Bram Boeckx, Hanne Vos, Damya Laoui, Emre Etlioglu et al. A pan-cancer blueprint of the heterogeneous tumor microenvironment revealed by single-cell profiling Cell Research 2020-09-01 [PMID: 32561858]

Bioinformatics

• Gene Symbol: TCF7
• Uniprot:
  • P36402(Human)