TCF-3/E2A Antibody (5G2) - Azide and BSA Free

Western Blot: TCF-3/E2A Antibody (5G2) [H00006929-M01] - TCF3 monoclonal antibody (M01), clone 5G2 Analysis of TCF3 expression in Hela S3 NE.

Sandwich ELISA: TCF-3/E2A Antibody (5G2) [H00006929-M01] - Detection limit for recombinant GST tagged TCF3 is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu, Rt
• Applications: WB, ELISA, ICC/IF
• Clone: 5G2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

TCF-3/E2A Antibody (5G2) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: TCF3 (NP_003191, 545 a.a. ~ 654 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EREKERRVANNARERLRVRDINEAFKELGRMCQLHLNSEKPQTKLLILHQAVSVILNLEQQVRERNLNPKAACLKRREEEKVSGVVGDPQMVLSAPHPGLSEAHNPAGHM
• Specificity: TCF3 - transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47)
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TCF3
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA
  • Western Blot
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.
• Control:

  HeLa Nuclear Cell Lysate

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for TCF-3/E2A Antibody (5G2) - Azide and BSA Free

• bHLHb21
• bHLHb21TCF-3
• Class B basic helix-loop-helix protein 21
• E2A immunoglobulin enhancer-binding factor E12/E47
• E2A
• E2AKappa-E2-binding factor
• Immunoglobulin enhancer-binding factor E12/E47
• Immunoglobulin transcription factor 1
• ITF1
• ITF1Transcription factor ITF-1
• Kappa-E2-binding factor
• MGC129647
• MGC129648
• Pan2
• TCF-3
• Tcfe2a
• transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47)
• Transcription factor 3
• transcription factor E2-alpha
• VDIR
• VDR interacting repressor

Background

TCF3 / E2A encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. This gene has been linked to sporatic cases of maturity-onset diabetes of the young. Two transcript variants encoding the same protein have been identified for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TCF-3/E2A Antibody (H00006929-M01)(1)

Publication using H00006929-M01

• Schmeisser MJ, Grabrucker AM, Bockmann J, et al. Synaptic cross-talk between N-methyl-D-aspartate receptors and LAPSER1-beta-catenin at excitatory synapses. J Biol Chem 284(42):29146-57. 2009-10-16 [PMID: 19703901]

Bioinformatics

• Gene Symbol: TCF3
• Entrez:
  • 6929(Human)
• OMIM:
  • 147141(Human)
• Uniprot:
  • NP_003191()