TCF-2/HNF-1 beta Antibody

Western Blot: TCF-2/HNF-1 beta Antibody [NB100-57093] - Staining of Human Liver lysate with antibody at 0.1 ug/mL (35ug protein in RIPA buffer). Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Ze
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

TCF-2/HNF-1 beta Antibody Summary

• Immunogen: Peptide with sequence C-QAYDRQKNPSKEER corresponding to internal region according to NP_001159395.1.
• Specificity: This antibody is expected to recognize both reported isoforms (NP_000449.1; NP_001159395.1). This product may cross-react with HNF1A.
• Predicted Species: Rat (100%), Porcine (100%), Bovine (100%), Zebrafish (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: HNF1B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.1 - 0.3 ug/mL
• Application Notes: WB: Approx 60 kDa band observed in Human Liver lysates (calculated MW of 61.3 kDa according to NP_000449.1).
• Theoretical MW: 61 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Human Liver Whole Tissue Lysate (Adult Whole Normal)
  TCF-2/HNF-1 beta Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TCF-2/HNF-1 beta Antibody

• FJHN
• hepatocyte nuclear factor 1-beta
• HNF1 beta A
• HNF-1 beta
• HNF1 homeobox B
• HNF1B
• HNF-1B
• HNF1beta
• HNF-1-beta
• HNF2
• Homeoprotein LFB3
• LFB3
• LF-B3
• MODY5
• MODY5HPC11
• TCF2
• TCF-2
• TCF2transcription factor 2, hepatic; LF-B3; variant hepatic nuclear factor
• Transcription factor 2
• transcription factor 2, hepatic
• Variant hepatic nuclear factor 1
• VHNF1

Background

TCF2 encodes transcription factor 2, a liver-specific factor of the homeobox-containing basic helix-turn-helix family. The TCF2 protein is believed to form heterodimers with another liver-specific member of this transcription factor family, TCF1; depending on the TCF2 isoform, the result may be to activate or inhibit transcription of target genes. Mutation of TCF2 that disrupts normal function has been identified as the cause of MODY5 (Maturity-Onset of Diabetes, Type 5). A third human transcript variant is believed to exist based on such a variant in the rat: however, to date such an mRNA species has not been isolated.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TCF-2/HNF-1 beta Antibody (NB100-57093)(2)

Publications using NB100-57093

• Miguel-Escalada I, Maestro MA, Balboa D et al. Pancreas agenesis mutations disrupt a lead enhancer controlling a developmental enhancer cluster Developmental cell 2022-08-22 [PMID: 35998583] (ICC/IF, Human)
  
  Details:
  Supplementary figure 5
• Terasawa K, Toyota M, Sagae S et al. Epigenetic inactivation of TCF2 in ovarian cancer and various cancer cell lines. Br J Cancer 2006-03-27 [PMID: 16479257]

Bioinformatics

• Gene Symbol: HNF1B
• Entrez:
  • 6928(Human)
  • 21410(Mouse)
  • 25640(Rat)
• Uniprot:
  • P35680()