TCF-12/HTF4 Antibody (690129)

TCF-12/HTF4 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human TCF-12/HTF4 Monoclonal Antibody (Catalog # MAB69281) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: ICC/IF
• Clone: 690129
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

TCF-12/HTF4 Antibody (690129) Summary

• Immunogen: E. coli-derived recombinant human TCF-12/HTF4
  Met108-Glu521
  Accession # Q99081
• Specificity: Detects human TCF-12/HTF4 in direct ELISAs. In direct ELISAs, 75%‑100% cross-reactivity with recombinant human (rh) TCF-3 and no cross-reactivity with rhTCF-1, -2, -4, or rhStathmin-2 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TCF12
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TCF-12/HTF4 Antibody (690129)

• BHLHB20
• bHLHb20E-box-binding protein
• Class B basic helix-loop-helix protein 20
• HEB
• HEBDNA-binding protein HTF4
• helix-loop-helix transcription factor 4
• HsT17266
• HTF4
• HTF4TCF-12
• TCF12
• TCF-12
• transcription factor 12
• Transcription factor HTF-4

Background

TCF-12 (transcription factor 12), also called HTF4, bHLHb20 and HEB, is a 682 amino acid (aa) DNA binding protein of the E protein family of helix-loop-helix (HLH) transcription factors. E proteins, including TCF-12 and E2A, mediate lymphocyte differentiation and also function in neurogenesis. Homo-oligomerization is required for activity; hetero-oligomers are also formed. The region of human TCF-12 used as an immunogen excludes the HLH domain and shares 94% aa sequence identity with mouse and rat TCF-12. Potential isoforms of 706 and 648 aa contain an insertion of 24 aa after aa 396, while isoforms of 648, 624 and 512 aa show alternate N‑terminal sequences that merge with the 682 aa form at aa 75, 75 and 194, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TCF12
• Uniprot:
  • Q99081()