TBR1 Antibody

Western Blot: TBR1 Antibody [NBP3-13178] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with TBR1 antibody (NBP3-13178) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: TBR1 Antibody [NBP3-13178] - TBR1 antibody detects TBR1 protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TBR1 stained by TBR1 antibody (NBP3-13178) diluted at 1:500. Red: alpha Tubulin, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:500. Blue: Fluoroshield with DAPI. Scale bar= 10um.

Immunohistochemistry-Paraffin: TBR1 Antibody [NBP3-13178] - TBR1 antibody detects TBR1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. TBR1 stained by TBR1 antibody (NBP3-13178) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: TBR1 Antibody [NBP3-13178] - TBR1 antibody detects TBR1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. TBR1 stained by TBR1 antibody (NBP3-13178) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

• Reactivity: Mu, Rt, Hu, Bv
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

TBR1 Antibody Summary

• Description: Centrifuge briefly prior to opening.
• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of mouse TBR1. The exact sequence is proprietary.
• Predicted Species: Human (99%), Bovine (99%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TBR1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 74 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol, 1% BSA
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for TBR1 Antibody

• MGC141978
• T-box brain protein 1
• T-box, brain, 1
• TBR-1
• T-brain-1
• TES-56

Background

TBR1 - T-box, brain, 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TBR1