TBP like protein TLP Antibody

Western Blot: TBP like protein TLP Antibody [NB100-1101] - (2ug/ml) of Jurkat lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

TBP like protein TLP Antibody Summary

• Description: Novus Biologicals Goat TBP like protein TLP Antibody (NB100-1101) is a polyclonal antibody validated for use in WB and ELISA. Anti-TBP like protein TLP Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-QIYPFVFESRKEIL corresponding to C-Terminus according to NP_004856.1.
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TBPL1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:4000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 20 kDa band observed in Jurkat lysate (predicted MW of 20 kDa band according to NP_004856).
• Control:

  TBP like protein TLP Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TBP like protein TLP Antibody

• 21 kDa TBP-like protein
• STUD21-kDA TBP-like protein
• TATA box binding protein-related factor 2
• TATA box-binding protein-like protein 1
• TATA box-binding protein-related factor 2
• TBP-like 1
• TBP-like factor
• TBP-like protein 1
• TBP-related factor 2
• TBP-related protein
• TLFMGC:8389
• TLP21
• TLPMGC:9620
• TRF2Second TBP of unique DNA protein
• TRP

Background

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes a protein that serves the same function as TBP and substitutes for TBP at some promoters that are not recognized by TFIID. It is essential for spermiogenesis and believed to be important in expression of developmentally regulated genes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TBP like protein TLP Antibody (NB100-1101)(1)

Publication using NB100-1101

• Ohbayashi T, Kishimoto T, Makino Y et al. Isolation of cDNA, chromosome mapping, and expression of the human TBP-like protein. Biochem Biophys Res Commun 1999-02-05 [PMID: 10082669]

Bioinformatics

• Gene Symbol: TBPL1
• Entrez:
  • 9519(Human)
  • 237336(Mouse)
• Uniprot:
  • NP_004856.(Human)