TBLR1 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: TBLR1 Antibody [NBP1-89880] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.

Analysis in human cerebral cortex and skeletal muscle tissues using NBP1-89880 antibody. Corresponding TBL1XR1 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: TBLR1 Antibody [NBP1-89880] - Staining of human prostate shows moderate to strong nuclear positivity in glandular cells.

Staining of human cerebral cortex shows moderate to strong nuclear positivity in neurons.

Staining of human skeletal muscle shows weak to moderate nuclear positivity in myocytes.

Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.

Simple Western: TBLR1 Antibody [NBP1-89880] - Simple Western lane view shows a specific band for TBLR1 in 0.2 mg/ml of RT-4 (left) and U-2 OS (right) lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Simple Western: TBLR1 Antibody [NBP1-89880] - Electropherogram image of the corresponding Simple Western lane view. TBLR1 antibody was used at 1:50 dilution on RT-4 and U-2 OS lysate(s) respectively.

Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line RT-4

ChIP-Exo-Seq composite graph for Anti-TBL1XR1 (NBP1-89880) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western, ICC/IF, IHC, ChIP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

TBLR1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit TBLR1 Antibody - BSA Free (NBP1-89880) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and Simple Western. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: RPIESLSLIDAVMPDVVQTRQQAYRDKLAQQQAAAAAAAAAAASQQGSAKNGENTANGEENGAHTIANNHTDMMEVDGDVEIPPNKAVVLRGHE
• Predicted Species: Mouse (96%), Rat (97%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TBL1XR1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation-exo-Seq 1-10ug per reaction
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Simple Western 1:50
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: Tested in RT-4 and U-2 OS lysates, separated by Size, antibody dilution of 1:50, apparent MW was 62 kDa.
• Control Peptide:

  TBLR1 Protein (NBP1-89880PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for TBLR1 Antibody - BSA Free

• C21
• DC42
• F-box-like/WD repeat-containing protein TBL1XR1
• FLJ12894
• IRA1Transducin beta-like 1X-related protein 1
• Nuclear receptor corepressor/HDAC3 complex subunit TBLR1
• nuclear receptor co-repressor/HDAC3 complex subunit
• TBLR1TBL1-related protein 1
• transducin (beta)-like 1 X-linked receptor 1
• transducin (beta)-like 1X-linked receptor 1

Background

TBLR1 is encoded by this gene has sequence similarity with members of the WD40 repeat-containing protein family. The WD40 group is a large family of proteins, which appear to have a regulatory function. It is believed that the WD40 repeats mediate protein-protein interactions and members of the family are involved in signal transduction, RNA processing, gene regulation, vesicular trafficking, cytoskeletal assembly and may play a role in the control of cytotypic differentiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TBL1XR1