TBLR1 Antibody

Images

 
Biological Strategies: Western Blot: TBLR1 Antibody [NB600-270] - Detection of Human and Mouse TBLR1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN ...read more
Immunohistochemistry-Paraffin: TBLR1 Antibody [NB600-270] - FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- TBLR1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunoprecipitation: TBLR1 Antibody [NB600-270] - Detection of human TBLR1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P, IP, ChIP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

TBLR1 Antibody Summary

Immunogen
A synthetic peptide that maps to a region between residues 100 and 150 of human TBL1-related Protein 1 using the numbering given in SwissProt entry Q9BZK7 (GeneID 79718).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TBL1XR1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000 - 1:10000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation 2-10 ug/mg lysate
  • Chromatin Immunoprecipitation (ChIP)
Application Notes
Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. In some cases, the antibody may be diluted further than indicated. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Use in chromatin immunoprecipitation reported in scientific literature (PMID: 31428774).
Control
HeLa Nuclear Cell Lysate
Publications
Read Publications using
NB600-270 in the following applications:

  • 1 publication
  • IP
    2 publications
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TBLR1 Antibody

  • C21
  • DC42
  • F-box-like/WD repeat-containing protein TBL1XR1
  • FLJ12894
  • IRA1Transducin beta-like 1X-related protein 1
  • Nuclear receptor corepressor/HDAC3 complex subunit TBLR1
  • nuclear receptor co-repressor/HDAC3 complex subunit
  • TBLR1TBL1-related protein 1
  • transducin (beta)-like 1 X-linked receptor 1
  • transducin (beta)-like 1X-linked receptor 1

Background

The protein encoded by this gene has sequence similarity with members of the WD40 repeat-containing protein family. The WD40 group is a large family of proteins, which appear to have a regulatory function. It is believed that the WD40 repeats mediate protein-protein interactions and members of the family are involved in signal transduction, RNA processing, gene regulation, vesicular trafficking, cytoskeletal assembly and may play a role in the control of cytotypic differentiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TBLR1 Antibody (NB600-270)(10)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ChIP, IP, WB.


Filter By Application
ChIP
(1)
IP
(2)
WB
(1)
All Applications
Filter By Species
Human
(3)
Mouse
(1)
All Species
Showing Publications 1 - 10 of 10.
Publications using NB600-270 Applications Species
Sundaram A. Linking metabolic syndrome and pancreatic cancer through transcriptional regulation and secreted factors from white adipocytes Thesis 2018 (Human) Human
Rohm M, Sommerfeld A, Strzoda D et al. Transcriptional Cofactor TBLR1 Controls Lipid Mobilization in White Adipose Tissue Cell Metab 2013 Mar 12 [PMID: 23499424] (WB, Mouse) WB Mouse
Ramadoss S, Li J, Ding X et al. Transducin beta-like protein 1 recruits nuclear factor ?B to the target gene promoter for transcriptional activation. Mol Cell Biol 2011 Mar [PMID: 21189284]
Dimitrova YN, Li J, Lee YT et al. Direct ubiquitination of beta-catenin by Siah-1 and regulation by the exchange factor TBL1. J Biol Chem 2010 Apr [PMID: 20181957]
Tabata T, Kokura K, Ten Dijke P et al. Ski co-repressor complexes maintain the basal repressed state of the TGF-beta target gene, SMAD7, via HDAC3 and PRMT5. Genes Cells 2009 Jan [PMID: 19032343]
Li J, Wang CY. TBL1-TBLR1 and beta-catenin recruit each other to Wnt target-gene promoter for transcription activation and oncogenesis. Nat Cell Biol 2008 Feb [PMID: 18193033]
Legrand N, Bretscher CL, Zielke S et al. PPAR beta/delta recruits NCOR and regulates transcription reinitiation bioRxiv Jul 2 2019
Legrand N, Bretscher CL, Zielke S et al. PPAR beta/delta recruits NCOR and regulates transcription reinitiation of ANGPTL4 Nucleic Acids Res. Aug 20 2019 [PMID: 31428774] (ChIP) ChIP
Legrand N, Bretscher CL, Zielke S et al. PPARbeta/delta controls Mediator recruitment and transcription initiation bioRxiv Jul 11 1905 (IP, Human) IP Human
Legrand N, Bretscher CL, Zielke S et al. PPAR beta/delta controls Mediator recruitment and transcription initiation bioRxiv Jan 24 2019 (IP, Human) IP Human

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for TBLR1 Antibody (NB600-270) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB600-270AF350
Alexa Fluor 405 NB600-270AF405
Alexa Fluor 488 NB600-270AF488
Alexa Fluor 532 NB600-270AF532
Alexa Fluor 594 NB600-270AF594
Alexa Fluor 647 NB600-270AF647
Alexa Fluor 700 NB600-270AF700
Alexa Fluor 750 NB600-270AF750
Biotin NB600-270B
DyLight 350 NB600-270UV
DyLight 405 NB600-270V
DyLight 488 NB600-270G
DyLight 550 NB600-270R
DyLight 594 NB600-270DL594
DyLight 650 NB600-270C
DyLight 680 NB600-270FR
DyLight 755 NB600-270IR
FITC NB600-270F
HRP NB600-270H
Janelia Fluor 549 NB600-270JF549
Janelia Fluor 646 NB600-270JF646

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Bioinformatics

Gene Symbol TBL1XR1
Entrez
Uniprot