TBLR1 Antibody - BSA Free

Western Blot: TBLR1 Antibody [NB600-270] - Detection of Human and Mouse TBLR1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TBLR1 antibody NB600-270 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Immunohistochemistry-Paraffin: TBLR1 Antibody [NB600-270] - FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- TBLR1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Immunoprecipitation: TBLR1 Antibody [NB600-270] - Detection of human TBLR1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TBLR1 antibody NB600-270 (lot NB600-270-2) used for IP at 6 ug per reaction. TBLR1 was also immunoprecipitated by a previous lot of this antibody (lot NB600-270-1). For blotting immunoprecipitated TBLR1, NB600-270 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

• Reactivity: Hu, Mu
• Applications: WB, IHC, IP, ChIP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

TBLR1 Antibody - BSA Free Summary

• Immunogen: A synthetic peptide that maps to a region between residues 100 and 150 of human TBL1-related Protein 1 using the numbering given in SwissProt entry Q9BZK7 (GeneID 79718).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TBL1XR1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation 2-10 ug/mg lysate
  • Western Blot 1:2000 - 1:10000
• Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. In some cases, the antibody may be diluted further than indicated. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Use in chromatin immunoprecipitation reported in scientific literature (PMID: 31428774).
• Control:

  HeLa Nuclear Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TBLR1 Antibody - BSA Free

• C21
• DC42
• F-box-like/WD repeat-containing protein TBL1XR1
• FLJ12894
• IRA1Transducin beta-like 1X-related protein 1
• Nuclear receptor corepressor/HDAC3 complex subunit TBLR1
• nuclear receptor co-repressor/HDAC3 complex subunit
• TBLR1TBL1-related protein 1
• transducin (beta)-like 1 X-linked receptor 1
• transducin (beta)-like 1X-linked receptor 1

Background

The protein encoded by this gene has sequence similarity with members of the WD40 repeat-containing protein family. The WD40 group is a large family of proteins, which appear to have a regulatory function. It is believed that the WD40 repeats mediate protein-protein interactions and members of the family are involved in signal transduction, RNA processing, gene regulation, vesicular trafficking, cytoskeletal assembly and may play a role in the control of cytotypic differentiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TBLR1 Antibody (NB600-270)(9)

Publications using NB600-270

• Rohm M, Sommerfeld A, Strzoda D et al. Transcriptional Cofactor TBLR1 Controls Lipid Mobilization in White Adipose Tissue Cell Metab 2013-03-12 [PMID: 23499424] (WB, Mouse)
• Ramadoss S, Li J, Ding X et al. Transducin beta-like protein 1 recruits nuclear factor ?B to the target gene promoter for transcriptional activation. Mol Cell Biol 2011-03-01 [PMID: 21189284]
• Dimitrova YN, Li J, Lee YT et al. Direct ubiquitination of beta-catenin by Siah-1 and regulation by the exchange factor TBL1. J Biol Chem 2010-04-01 [PMID: 20181957]
• Tabata T, Kokura K, Ten Dijke P et al. Ski co-repressor complexes maintain the basal repressed state of the TGF-beta target gene, SMAD7, via HDAC3 and PRMT5. Genes Cells 2009-01-01 [PMID: 19032343]
• Li J, Wang CY. TBL1-TBLR1 and beta-catenin recruit each other to Wnt target-gene promoter for transcription activation and oncogenesis. Nat Cell Biol 2008-02-01 [PMID: 18193033]
• Sundaram A. Linking metabolic syndrome and pancreatic cancer through transcriptional regulation and secreted factors from white adipocytes Thesis 2018-01-01 (Human)
• Legrand N, Bretscher CL, Zielke S et al. PPARbeta/delta controls Mediator recruitment and transcription initiation bioRxiv 1905-07-11 (IP, Human)
• Mastrototaro G, Zaghi M, Massimino L et al. TBL1XR1 Ensures Balanced Neural Development Through NCOR Complex-Mediated Regulation of the MAPK Pathway Frontiers in Cell and Developmental Biology 2021-02-23 [PMID: 33708771] (WB, Mouse)
• Legrand N, Bretscher CL, Zielke S et al. PPAR beta/delta recruits NCOR and regulates transcription reinitiation of ANGPTL4 Nucleic Acids Res. 2019-08-20 [PMID: 31428774] (Chemotaxis)

Bioinformatics

• Gene Symbol: TBL1XR1
• Entrez:
  • 79718(Human)
• Uniprot:
  • Q9BZK7(Human)