TAZ/WWTR1 Antibody (CL0371) - BSA Free

Immunohistochemistry-Paraffin: TAZ/WWTR1 Antibody (CL0371) [NBP2-52926] - Staining in human placenta and lymph node tissues. Corresponding WWTR1 RNA-seq data are presented for the same tissues.

Western Blot: TAZ/WWTR1 Antibody (CL0371) [NBP2-52926] - Analysis in EFO-21 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-WWTR1 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.

Immunocytochemistry/Immunofluorescence: TAZ/WWTR1 Antibody (CL0371) [NBP2-52926] - Staining in SKMEL cell line with Anti-WWTR1 monoclonal antibody, showing spotty nuclear staining in green. Microtubule probes are visualized in red (where available). Antibody staining is shown in green.

Western Blot: TAZ/WWTR1 Antibody (CL0371) [NBP2-52926] - Lane 1: Marker [kDa], Lane 2: Human cell line U-251 MG

Immunohistochemistry-Paraffin: TAZ/WWTR1 Antibody (CL0371) [NBP2-52926] - Staining of human breast cancer shows moderate to strong nuclear positivity in tumor cells.

Immunohistochemistry-Paraffin: TAZ/WWTR1 Antibody (CL0371) [NBP2-52926] - Staining of human placenta shows strong nuclear and some cytoplasmic positivity in endothelial cells.

Immunohistochemistry-Paraffin: TAZ/WWTR1 Antibody (CL0371) [NBP2-52926] - Staining of human kidney shows strong nuclear and moderate cytoplasmic positivity in cells in tubules and in glomeruli.

Immunohistochemistry-Paraffin: TAZ/WWTR1 Antibody (CL0371) [NBP2-52926] - Staining of human lymph node shows no nuclear positivity in lymphoid cells as expected.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, Mycoplasma
• Clone: CL0371
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

TAZ/WWTR1 Antibody (CL0371) - BSA Free Summary

• Description: Novus Biologicals Mouse TAZ/WWTR1 Antibody (CL0371) - BSA Free (NBP2-52926) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed using a recombinant protein derived from Q9GZV5, with the exact immunogen sequence remaining proprietary.
• Predicted Species: Mouse (96%), Rat (96%). Backed by our 100% Guarantee.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: WWTR1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Knockdown Validated
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  TAZ/WWTR1 Recombinant Protein Antigen (NBP2-52926PEP)

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for TAZ/WWTR1 Antibody (CL0371) - BSA Free

• FLJ27004
• FLJ45718
• TAZ
• TAZDKFZp586I1419
• transcriptional co-activator with PDZ-binding motif
• Transcriptional coactivator with PDZ-binding motif
• WW domain containing transcription regulator 1
• WW domain-containing transcription regulator protein 1
• WWTR1

Background

TAZ is a YAP-related protein that contains a WW domain and transcriptional activation region. TAZ plays a role in mesenchymal stem cell differentiation. The antibody was designed in such a way that should generate a TAZ specific-reagent able to differentiate between YAP and TAZ proteins in cells and tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for TAZ/WWTR1 Antibody (NBP2-52926). (Showing 1 - 1 of 1 FAQ).

1. I want to know which TAZ primary antibody is best produced for WB (human and mouse)!
   • I would recommend this TAZ antibody: NB110-58359. This is also comes in a sample size.

Bioinformatics

• Gene Symbol: WWTR1