TAp63 Antibody (TAp63-4.1) - BSA Free

Western Blot: TAp63 Antibody (TAp63-4.1) [NBP3-11703] - Analysis, confirming specificity of mouse monoclonal antibody TAp63-4.1 for TAp63 isoform of p63 protein. Cell lysates from particular transfectants of TAp63alpha, TAp63gamma, deltaNp63alpha, TAp73alpha, and deltaNp73alpha were analysed.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IP
• Clone: TAp63-4.1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

TAp63 Antibody (TAp63-4.1) - BSA Free Summary

• Immunogen: Recombinant human p63
• Epitope: Target epitope is LSDPMW.
• Specificity: The mouse monoclonal antibody TAp63-4.1 recognizes TAp63. This antibody does not bind to deltaNp63 isoform of p63.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: TP63
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot 1 ug/ml

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• Preservative: 15mM Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for TAp63 Antibody (TAp63-4.1) - BSA Free

• p63 Transactivation Domain Isoform
• Transactivation p63
• Transactivation Tumor protein 63

Background

The p53 family of proteins includes three members, p53, p63, and p73. The protein p63 is encoded by TP63 gene, which gives rise to protein isoforms with different properties and functions due to the presence (TAp63) or absence (deltaNp63) of an N-terminal transactivation domain. Immunohistochemistry of p63 has a clinical relevance for certain tumor types, but investigations have been hampered by a lack of well characterized antibodies that are specific for p63, do not cross-react with the related p53 and p73 proteins, and allow for discrimination between p63 isoforms TAp63 and deltaNp63 with opposite functional properties.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TP63