![Western Blot: Tankyrase 1 Antibody (PSH0-51) [NBP3-33033] - Western blot analysis of Tankyrase 1 on different lysates with Rabbit anti-Tankyrase 1 antibody (NBP3-33033) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: MCF7 cell lysate Lane 4: Raji cell lysate Lane 5: HepG2 cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: PC-12 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 142 kDa Observed band size: 150 kDa Exposure time: 17 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33033) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33033_rabbit-tankyrase-1-mab-psh0-51-28820241249345.jpg "Western Blot: Tankyrase 1 Antibody (PSH0-51) [NBP3-33033] - Western blot analysis of Tankyrase 1 on different lysates with Rabbit anti-Tankyrase 1 antibody (NBP3-33033) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: MCF7 cell lysate Lane 4: Raji cell lysate Lane 5: HepG2 cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: PC-12 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 142 kDa Observed band size: 150 kDa Exposure time: 17 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33033) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB |
| Clone | PSH0-51 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Description | Novus Biologicals Rabbit Tankyrase 1 Antibody (PSH0-51) (NBP3-33033) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant protein within human Tankyrase 1 aa 151-650/1,327. (Uniprot: O95271) |
| Localization | Cytoplasm, Golgi apparatus membrane, cytoskeleton, microtubule organizing center, centrosome, Nucleus, nuclear pore complex, Chromosome, telomere, cytoskeleton, spindle pole. |
| Source | HEK293 |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | TNKS |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 142 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH7.4), 0.1% BSA and 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Tankyrase 1 Antibody (NBP3-33033)Find related products by research area.
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| Gene Symbol | TNKS |
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