RTEL1 Antibody

Images

 
Western Blot: RTEL1 Antibody [NBP2-22360] - WB analysis of RTEL1 in HeLa cell lysate.
Immunocytochemistry/ Immunofluorescence: RTEL1 Antibody [NBP2-22360] - RTEL1 antibody was tested in Hela cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 ...read more
Immunohistochemistry: RTEL1 Antibody [NBP2-22360] - IHC analysis of RTEL1 in mouse intestine.
Western Blot: RTEL1 Antibody [NBP2-22360] - WB analysis of RTEL1 in mouse testis lysate.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.26 mg/ml

RTEL1 Antibody Summary

Immunogen
A genomic peptide made to an internal region of the human RTEL1 protein (within residues 800-1000). [Swiss-Prot Q9NZ71]
Localization
Nucleus
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RTEL1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000 - 1:10000
  • Immunocytochemistry/Immunofluorescence 1:250 - 1:500
  • Immunohistochemistry 1:300
  • Immunohistochemistry-Paraffin 1:300
  • Immunoprecipitation
Application Notes
In Immunohistochemistry and ICC/IF nuclear staining was observed. Formulin fixation is recommended for ICC/IF.
Positive Control
HeLa Lysate (NB800-PC1)
Reviewed Applications
Read 1 Review rated 4
using
NBP2-22360 in the following applications:

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.26 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RTEL1 Antibody

  • C20orf41
  • regulator of telomere elongation helicase 1
  • regulator of telomere length

Background

RTEL1 (Regulator of Telomere Length 1) is a nuclear iron-sulfur cluster containing ATP-dependent DNA helicase essential to genomic stability. RTEL1 contains 1 helicase ATP-binding domain and it belongs to helicase family, RAD3/XPD subfamily. Originally discovered in mouse, RTEL1 is a dominant factor controlling the regulation of telomeric length and genomic integrity, and recent evidence suggest that RTEL1 is preferentially recruited to, or exerts greater activity at genome's G-rich regions. In DNA double-strand breaks (DSBs) repair, RTEL1 disrupts D-loops in vitro and promotes synthesis-dependent strand annealing (SDSA) in vivo to direct DNA DSBs into non-crossover outcomes during mitotic repair. RTEL1 activity is also essential to meiotic recombination regulation as shown in C elegans. RTEL1's multiple roles in genomic stability maintenance and recombination regulation suggests it as a tumor suppressor. Indeed, RTEL1 mutation represents a risk factor for glioma and it is overexpressed in gastrointestinal cancers. Human RTEL1 deficiency caused Hoyeraal-Hreidarsson syndrome (HHS), a severe variant of Dyskeratosis Congenita (DC), characterized by early onset bone marrow failure, immunodeficiency and developmental defects.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RTEL1 Antibody (NBP2-22360) (0)

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Review for RTEL1 Antibody (NBP2-22360) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP2-22360:
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(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Fernanda gutierrez rodrigues
WB Human 09/06/2017
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Summary

ApplicationWestern Blot
Sample Tested293T cell lysate
SpeciesHuman
LotA062817H

Comments

CommentsOverexpression of RTEL1 in 293T cells

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for RTEL1 Antibody (NBP2-22360). (Showing 1 - 1 of 1 FAQ).

  1. I would like to know which protocol have you used to produce the image of RTEL1 in intestine sections. Which retrieval conditions are you using?
    • For NBP2-22360 we used Citrate Buffer pH 6.0 for the antigen retrieval. For our in-house testing, we used a dilution of 1:250; however, depending on your experimental conditions, you will need to optimize.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP2-22360AF405
Alexa Fluor 488 Labeled NBP2-22360AF488
Alexa Fluor 647 Labeled NBP2-22360AF647
Alexa Fluor 700 Labeled NBP2-22360AF700
Biotin Labeled NBP2-22360B
DyLight 350 Labeled NBP2-22360UV
DyLight 405 Labeled NBP2-22360V
DyLight 488 Labeled NBP2-22360G
DyLight 550 Labeled NBP2-22360R
DyLight 650 Labeled NBP2-22360C
DyLight 680 Labeled NBP2-22360FR
DyLight 755 Labeled NBP2-22360IR
HRP Labeled NBP2-22360H

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Calculators

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Recent Reviews

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5
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4
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2
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1
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Fernanda gutierrez rodrigues
09/06/2017
Application: WB
Species: Human

Bioinformatics

Gene Symbol RTEL1
Entrez
Uniprot