Western Blot: RTEL1 Antibody [NBP2-22360] - WB analysis of RTEL1 in HeLa cell lysate.
Immunocytochemistry/ Immunofluorescence: RTEL1 Antibody [NBP2-22360] - RTEL1 antibody was tested in Hela cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 ...read more
Immunohistochemistry: RTEL1 Antibody [NBP2-22360] - IHC analysis of RTEL1 in mouse intestine.
Western Blot: RTEL1 Antibody [NBP2-22360] - WB analysis of RTEL1 in mouse testis lysate.
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
Immunogen affinity purified
Alternate Names for RTEL1 Antibody
regulator of telomere elongation helicase 1
regulator of telomere length
RTEL1 (Regulator of Telomere Length 1) is a nuclear iron-sulfur cluster containing ATP-dependent DNA helicase essential to genomic stability. RTEL1 contains 1 helicase ATP-binding domain and it belongs to helicase family, RAD3/XPD subfamily. Originally discovered in mouse, RTEL1 is a dominant factor controlling the regulation of telomeric length and genomic integrity, and recent evidence suggest that RTEL1 is preferentially recruited to, or exerts greater activity at genome's G-rich regions. In DNA double-strand breaks (DSBs) repair, RTEL1 disrupts D-loops in vitro and promotes synthesis-dependent strand annealing (SDSA) in vivo to direct DNA DSBs into non-crossover outcomes during mitotic repair. RTEL1 activity is also essential to meiotic recombination regulation as shown in C elegans. RTEL1's multiple roles in genomic stability maintenance and recombination regulation suggests it as a tumor suppressor. Indeed, RTEL1 mutation represents a risk factor for glioma and it is overexpressed in gastrointestinal cancers. Human RTEL1 deficiency caused Hoyeraal-Hreidarsson syndrome (HHS), a severe variant of Dyskeratosis Congenita (DC), characterized by early onset bone marrow failure, immunodeficiency and developmental defects.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for RTEL1 Antibody (NBP2-22360). (Showing 1 - 1 of 1 FAQ).
I would like to know which protocol have you used to produce the image of RTEL1 in intestine sections. Which retrieval conditions are you using?
For NBP2-22360 we used Citrate Buffer pH 6.0 for the antigen retrieval. For our in-house testing, we used a dilution of 1:250; however, depending on your experimental conditions, you will need to optimize.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.