TAK1 [p Ser439] Antibody (SR2176) - BSA Free

Western Blot: TAK1 [p Ser 439] Antibody (SR2176) [NBP3-21597] - Western blot analysis using NBP3-21597 in (1) HeLa treated with Calyculin A + IL-1 beta cell lysate; (2) Untreated.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IP
• Clone: SR2176
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

TAK1 [p Ser439] Antibody (SR2176) - BSA Free Summary

• Description: Novus Biologicals Rabbit TAK1 [p Ser439] Antibody (SR2176) - BSA Free (NBP3-21597) is a recombinant monoclonal antibody validated for use in WB and IP. Anti-TAK1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthesized peptide derived from human phospho-TAK1 [Ser439] (Uniprot #: O43318)
• Modification: p Ser439
• Specificity: Detects endogenous levels of total phospho-TAK1 [Ser439]
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: MAP3K7
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1:50
  • Western Blot 1:500-1:2000
• Theoretical MW: 72 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, 50% glycerol.
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for TAK1 [p Ser439] Antibody (SR2176) - BSA Free

• EC 2.7.11
• EC 2.7.11.25
• MAP3K7
• MEKK7
• mitogen-activated protein kinase kinase kinase 7
• TAK1
• TAK1/MAP3K7
• TAK1TGF1a
• TGF1a
• TGF-beta activated kinase 1
• TGF-beta-activated kinase 1
• Transforming growth factor-beta-activated kinase 1

Background

TAK1 (or MAP3K7) was shown to participate in regulation of transcription by transforming growth factor beta (TGF beta). TAK1 is stimulated in response to TGF beta and bone morphogenetic protein. These results suggest that TAK1 functions as a mediator in the signaling pathway of TGF beta superfamily members. TAB1 and TAB2 are TAK1 binding proteins that may function as activators of the TAK1 (TGF b activated kinase 1) MAPKKK in TGF b signal transduction. TAB1 induced TAK1 activation promoted the dissociation of active forms of IKKa and IKK b from active TAK1, whereas the IKK mutants remained to interact with active TAK1. TNF a activated endogenous TAK1, and the kinase negative TAK1 acted as a dominant negative inhibitor against TNF a induced NFkB activation. TAK1 was suggested to act as a regulatory kinase of IKKs.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TAK1 Antibody (NBP3-21597)(1)

Publication using NBP3-21597

• Pierre-Marie Boutanquoi, Amira Sayed Khan, Lidia Cabeza, Lucas Jantzen, Thomas Gautier, Semen Yesylevskyy, Christophe Ramseyer, David Masson, Vincent Van Waes, Aziz Hichami, Naim Akhtar Khan A novel fatty acid analogue triggers CD36–GPR120 interaction and exerts anti-inflammatory action in endotoxemia Cellular and Molecular Life Sciences 2024-04-10 [PMID: 38598021]

Bioinformatics

• Gene Symbol: MAP3K7