MKK4/MEK4 Antibody (OTI8A8)

Images

 
Western Blot: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Analysis of extracts (35ug) from 9 different cell lines by using anti-MEK4 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY MEK4.
Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Staining of paraffin-embedded mouse spleen tissue using MKK4/MEK4 antibody (OTI8A8). Image from verified customer review.
Flow Cytometry: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MEK4 antibody, and then ...read more
Immunocytochemistry/ Immunofluorescence: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Staining of HepG2 cells using anti-MEK4 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-MEK4 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MEK4 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Staining of paraffin-embedded Human pancreas tissue using anti-MEK4 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Staining of paraffin-embedded Human thyroid tissue using anti-MEK4 mouse monoclonal antibody.
Immunoprecipitation: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - (Negative control: IP without adding anti-MAP2K4 antibody.). For each experiment, 500ul of DDK tagged MAP2K4 overexpression lysates (at 1:5 dilution with ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, CaSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, IP
Clone
OTI8A8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
2.36 mg/ml

Order Details

MKK4/MEK4 Antibody (OTI8A8) Summary

Immunogen
Full-length protein expressed in 293T cell transfected with human MAP2K4 expression vector
Specificity
This antibody is specific for Homo sapiens mitogen-activated protein kinase kinase 4 (MAP2K4).
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
MAP2K4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:50-100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Immunoprecipitation 2ug/500ul
  • Western Blot 1:500-1000
Theoretical MW
44.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-47839 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
2.36 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MKK4/MEK4 Antibody (OTI8A8)

  • c-Jun N-terminal kinase kinase 1
  • dual specificity mitogen-activated protein kinase kinase 4
  • JNK activating kinase 1
  • JNK-activated kinase 1
  • JNK-activating kinase 1
  • JNKK
  • JNKK1
  • JNKK1MAPK/ERK kinase 4
  • MAP kinase kinase 4
  • MAP2K4
  • MAPKK4
  • MEK4
  • MEK4MEK 4
  • mitogen-activated protein kinase kinase 4
  • MKK4
  • MKK4SAPK/ERK kinase 1
  • PRKMK4
  • PRKMK4MAPKK 4
  • SEK1
  • SERK1
  • SERK1EC 2.7.12.2

Background

This gene encodes a dual specificity protein kinase that belongs to the Ser/Thr protein kinase family. This kinase is a direct activator of MAP kinases in response to various environmental stresses or mitogenic stimuli. It has been shown to activate MAPK8/JNK1, MAPK9/JNK2, and MAPK14/p38, but not MAPK1/ERK2 or MAPK3/ERK3. This kinase is phosphorylated, and thus activated by MAP3K1/MEKK. The knockout studies in mice suggested the roles of this kinase in mediating survival signal in T cell development, as well as in the organogenesis of liver

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MKK4/MEK4 Antibody (NBP1-47839) (0)

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Review for MKK4/MEK4 Antibody (NBP1-47839) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP1-47839:
Filter by Applications
Immunohistochemistry-Paraffin
(1)
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(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin MKK4/MEK4 NBP1-47839
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5
reviewed by:
Layla Schwartz
Immunohistochemistry-Paraffin Mouse 12/21/2021
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSpleen tissue
SpeciesMouse
LotOTI8A8

Product General Protocols

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Video Protocols

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ICC/IF Video Protocol

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Secondary Antibodies

 

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Recent Reviews

5
5
1
4
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3
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2
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1
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Layla Schwartz
12/21/2021
Application: Immunohistochemistry-Paraffin
Species: Mouse

Bioinformatics

Gene Symbol MAP2K4
Entrez