TAB1 Antibody [Unconjugated] (AF3578)

Western blot shows lysates of WS‑1 human fetal skin fibroblast cell line and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse TAB1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3578) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TAB1 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

TAB1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse TAB1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3578) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu, Mu
• Applications: WB, ICC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

TAB1 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human TAB1
  Ser7-Glu159
  Accession # Q15750
• Specificity: Detects human and mouse TAB1.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TAB1

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL
  • Immunocytochemistry 5-15 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TAB1 Antibody [Unconjugated]

• MAP3K7IP1
• MAP3K7IP13'-Tab1
• MGC57664
• mitogen-activated protein kinase kinase kinase 7 interacting protein 1
• Mitogen-activated protein kinase kinase kinase 7-interacting protein 1
• TAB1
• TAK1-binding protein 1
• TGF-beta activated kinase 1/MAP3K7 binding protein 1
• TGF-beta-activated kinase 1 and MAP3K7-binding protein 1
• TGF-beta-activated kinase 1-binding protein 1
• transforming growth factor beta-activated kinase-binding protein 1

Background

TAK1-binding protein 1 (TAB1), also known as MAP3K7IP1, is an activator of the MAP3K TAK1. The C-terminal 68 amino acids of TAB1 are sufficient for binding and activation of TAK1, while a portion of the N-terminus acts as a dominant-negative inhibitor of TGF-beta, suggesting that TAB1 may function as a signaling intermediate between TGF-beta receptors and TAK1. TAB1 also promotes autophosphorylation and activation of the MAPK p38 alpha.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TAB1 Antibody (AF3578)(1)

Publication using AF3578

• Zhao D, Zheng HQ, Zhou Z The Fbw7 tumor suppressor targets KLF5 for ubiquitin-mediated degradation and suppresses breast cell proliferation. Cancer Res., 2010;70(11):4728-38. 2010 [PMID: 20484041] (ICC,, Human)

Bioinformatics

• Gene Symbol: TAB1
• Entrez:
  • 10454(Human)
  • 66513(Mouse)
  • 315139(Rat)
• Uniprot:
  • Q15750()