TAB1 Antibody [Unconjugated]

HAF109). A specific band was detected for TAB1 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." > HAF109). A specific band was detected for TAB1 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." title="Western blot shows lysates of WS-1 human fetal skin fibroblast cell line and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse TAB1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3578) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TAB1 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." />

Western blot shows lysates of WS-1 human fetal skin fibroblast cell line and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse TAB1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3578) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." > NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." title="TAB1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse TAB1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3578) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." />

TAB1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse TAB1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3578) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # <a class=

• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

TAB1 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human TAB1
  Ser7-Glu159
  Accession # Q15750
• Specificity: Detects human and mouse TAB1.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TAB1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TAB1 Antibody [Unconjugated]

• MAP3K7IP1
• MAP3K7IP13'-Tab1
• MGC57664
• mitogen-activated protein kinase kinase kinase 7 interacting protein 1
• Mitogen-activated protein kinase kinase kinase 7-interacting protein 1
• TAB1
• TAK1-binding protein 1
• TGF-beta activated kinase 1/MAP3K7 binding protein 1
• TGF-beta-activated kinase 1 and MAP3K7-binding protein 1
• TGF-beta-activated kinase 1-binding protein 1
• transforming growth factor beta-activated kinase-binding protein 1

Background

TAK1-binding protein 1 (TAB1), also known as MAP3K7IP1, is an activator of the MAP3K TAK1. The C-terminal 68 amino acids of TAB1 are sufficient for binding and activation of TAK1, while a portion of the N-terminus acts as a dominant-negative inhibitor of TGF-beta , suggesting that TAB1 may function as a signaling intermediate between TGF-beta receptors and TAK1. TAB1 also promotes autophosphorylation and activation of the MAPK p38 alpha .

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TAB1 Antibody (AF3578)(3)

Publications using AF3578

• Kong, Y;Lan, T;Wang, L;Gong, C;Lv, W;Zhang, H;Zhou, C;Sun, X;Liu, W;Huang, H;Weng, X;Cai, C;Peng, W;Zhang, M;Jiang, D;Yang, C;Liu, X;Rao, Y;Chen, C; BRD4-specific PROTAC inhibits basal-like breast cancer partially through downregulating KLF5 expression Oncogene 2024-08-20 [PMID: 39164524] (Immunohistochemistry, Human)
• Ortiz B, Driscoll A, Menon R et al. Chlamydia trachomatis antigen induces TLR4-TAB1-mediated inflammation, but not cell death, in maternal decidua cells American journal of reproductive immunology (New York, N.Y. : 1989) 12/9/2022 [PMID: 36495029]
• Zhao D, Zheng HQ, Zhou Z The Fbw7 tumor suppressor targets KLF5 for ubiquitin-mediated degradation and suppresses breast cell proliferation. Cancer Res., 2010-05-18;70(11):4728-38. 2010-05-18 [PMID: 20484041] (ICC, Human)

Bioinformatics

• Gene Symbol: TAB1
• Uniprot:
  • Q15750()