Tafazzin/TAZ Antibody

Western Blot: Tafazzin/TAZ Antibody [NB600-811] - (0.3ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Tafazzin/TAZ Antibody Summary

• Immunogen: Peptide with sequence C-HLKTQAEQLHNH corresponding to C-Terminus according to NP_000107.1, NP_851828.1, NP_851829.1, NP_851830.1.
• Specificity: This antibody is expected to recognise isoforms 1to 4 (NP_000107.1; NP_851828.1; NP_851829.1; NP_851830.1, respectively).
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TAFAZZIN
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.3 - 10 ug/ml
• Application Notes: WB: Approx. 28 kDa band observed in human heart lysates (calculated MW of 33.5 kDa band according to NP_000107.1, 30.2 kDa band according to NP_851828.1, 31.7 kDa band according to NP_851829.1 and 28.5 according to NP_851830.1).
• Control:

  Tafazzin/TAZ Overexpression Lysate
  Human Heart Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Tafazzin/TAZ Antibody

• BTHSEFE
• cardiomyopathy, dilated 3A (X-linked)
• CMD3A
• EFE2FLJ27390
• G4.5endocardial fibroelastosis 2
• LVNCX
• Protein G4.5
• tafazzin
• Taz1
• XAP-2

Background

The tafazzin protein plays a key role in Barth syndrome. Barth syndrome appears to be a disease of the mitochondria. Tafazzin protrudes into the intermembrane space from both the outer and inner membranes. The tafazzin protein may be associated with cardiolipin, the signature lipid of mitochondria, which has been implicated in other diseases, including those of the heart. Tafazzin may be important in repairing lipids, including cardiolipin, but it is not determined. Tafazzin's association with both the outer membrane and the inner membrane is consistent with its ability to modify a membrane component such as cardiolipin. This antibody is expected to recognise isoforms 1to 4 (NP_000107.1; NP_851828.1; NP_851829.1; NP_851830.1, respectively).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Tafazzin/TAZ Antibody (NB600-811)(1)

Publication using NB600-811

• Brandner K, Mick DU, Frazier AE et al. Taz1, an outer mitochondrial membrane protein, affects stability and assemblyof inner membrane protein complexes: implications for Barth Syndrome. Mol Biol Cell 2005-11-01 [PMID: 16135531]

Bioinformatics

• Gene Symbol: TAFAZZIN
• Entrez:
  • 6901(Human)
• Uniprot:
  • NP_000107.1(Human)