TAF10 Antibody - C-terminus

• Reactivity: Hu
• Applications: WB, DB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

TAF10 Antibody - C-terminus Summary

• Immunogen: A synthetic peptide corresponding to C-Terminal portion of human TAF10.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TAF10
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Dot Blot
  • ELISA
  • Western Blot
• Application Notes: This antibody has been tested for use in ELISA, Dot Blot, and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 21.7 kDa in size corresponding to TAF10 protein by western blotting in the appropriate cell lysate or extract.
  Dot blot: 1.0 ug/ml

Reactivity Notes

BLAST analysis was used to suggest cross reactivity with TAF10 protein from human and mouse (100% homology). Reactivity against homologues from other sources is not known.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for TAF10 Antibody - C-terminus

• 30kDa
• STAF28
• TAF10 RNA polymerase II, TATA box binding protein (TBP)-associated factor
• TAF2ATAFII-30
• TAF2Htranscription initiation factor TFIID 30 kD subunit
• TAFII30TAF(II)30
• TATA box binding protein (TBP)-associated factor, RNA polymerase II, H, 30kD
• Transcription initiation factor TFIID 30 kDa subunit
• transcription initiation factor TFIID subunit 10

Background

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the small subunits of TFIID that is associated with a subset of TFIID complexes. Studies with human and mammalian cells have shown that this subunit is required for transcriptional activation by the estrogen receptor, for progression through the cell cycle, and may also be required for certain cellular differentiation programs.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TAF10