TACE/ADAM17 Antibody - BSA Free

Western Blot: TACE/ADAM17 Antibody [NBP2-15281] - Wild-type (WT) and ADAM17 knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ADAM17 antibody [C2C3], C-term. HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Western Blot: TACE/ADAM17 Antibody [NBP2-15281] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ADAM17 antibody [C2C3], C-term diluted at 1:500.

Immunohistochemistry-Paraffin: TACE/ADAM17 Antibody [NBP2-15281] - Human breast carcinoma dilution: 1:250. ADAM17 antibody [C2C3], C-term dilution: 1:250. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: TACE/ADAM17 Antibody [NBP2-15281] - 30 ug K562 whole cell lysate/extract, 7.5% SDS-PAGE gel, dilution 1:500.

Western Blot: TACE/ADAM17 Antibody [NBP2-15281] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ADAM17 antibody [C2C3], C-term diluted at 1:500.

Western Blot: TACE/ADAM17 Antibody [NBP2-15281] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TACE/ADAM17 antibody [C2C3], C-term (NBP2-15281) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Western Blot: TACE/ADAM17 Antibody [NBP2-15281] - Wild-type (WT) and ADAM17 knockout (KO) HeLa (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ADAM17 antibody [C2C3], C-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, Po, Ca
• Applications: WB, IHC, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

TACE/ADAM17 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human TACE/ADAM17. The exact sequence is proprietary.
• Localization: Membrane
• Predicted Species: Porcine (93%), Canine (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ADAM17
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Knockout Validated
  • Western Blot 1:500-1:3000
• Theoretical MW: 93 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chicken (83%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 40% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for TACE/ADAM17 Antibody - BSA Free

• ADAM 17
• ADAM metallopeptidase domain 17
• ADAM metallopeptidase domain 18
• ADAM17
• ADAM18
• CD156b antigen
• CD156b
• CSVP
• disintegrin and metalloproteinase domain-containing protein 17
• EC 3.4.24.86
• MGC71942
• Snake venom-like protease
• TACE
• TACEcSVP
• TNF-alpha convertase
• TNF-alpha converting enzyme
• TNF-alpha-converting enzyme
• tumor necrosis factor, alpha, converting enzyme

Background

This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biologic processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene functions as a tumor necrosis factor-alpha converting enzyme; binds mitotic arrest deficient 2 protein; and also plays a prominent role in the activation of the Notch signaling pathway. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for TACE/ADAM17 Antibody (NBP2-15281)(3)

Publications using NBP2-15281

• Balasubramanian N, James TD, Selvakumar GP et al. Repeated ethanol exposure and withdrawal alters angiotensin-converting enzyme 2 expression in discrete brain regions: Implications for SARS-CoV-2 neuroinvasion Alcoholism: Clinical and Experimental Research 2023-02-01 [PMID: 36529893] (Western Blot, Immunohistochemistry-Paraffin, Human)
• Y Zheng, NZ Prince, LN Peralta Ma, S Ahmed, J Garssen, P Perez Pard, AD Kraneveld The Autism Spectrum Disorder-Associated Bacterial Metabolite p-Cresol Derails the Neuroimmune Response of Microglial Cells Partially via Reduction of ADAM17 and ADAM10 International Journal of Molecular Sciences, 2022-09-20;23(19):. 2022-09-20 [PMID: 36232346] (Western Blot, Immunohistochemistry-Paraffin, Human)
• Sharma A, Bender S, Zimmermann M et al. Secretome Signature Identifies ADAM17 as Novel Target for Radiosensitization of Non-Small Cell Lung Cancer. Clin. Cancer Res. 2016-04-13 [PMID: 27076628] (IHC-P, WB, Human)

FAQs for TACE/ADAM17 Antibody (NBP2-15281). (Showing 1 - 1 of 1 FAQs).

1. Our customer would like to ensure NBP2-15281 could recognize both active and inactive forms of human ADAM17. Would you please help confirm?
   • The immunogen which was used to generate our ADAM17 antibody with catalogue number NBP2-15281 was a recombinant fragment corresponding to a region within amino acids 668 and 824 of ADAM17 (Uniprot ID# P78536). These amino acids are found within the chain of the protein, rather than in the signal peptide (amino acids 1-17) or the pro-peptide (amino acids 18-214). Metalloproteinases such as ADAM17 are synthesized as pro-proteins that become active by proteolytic removal of the pro-domain. Since the immunogen sequence lies within the chain you would expect NBP2-15281 to recognise both the active and inactive forms of the protein, although we do not have any specific testing data relating to this.

Bioinformatics

• Gene Symbol: ADAM17
• Uniprot:
  • P78536()