T-bet/TBX21 Antibody (1087415) [Unconjugated]

T‑bet/TBX21 was detected in immersion fixed paraffin-embedded sections of mouse spleen using Rat Anti-Mouse T‑bet/TBX21 Monoclonal Antibody (Catalog # MAB11627) at 5 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using the HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005) and counterstained with hematoxylin (blue). Specific staining was localized to the nucleus. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Western Blot shows lysates of HT‑2 mouse T cell line. PVDF membrane was probed with 2 µg/ml of Rat Anti-Mouse T‑bet/TBX21 Monoclonal Antibody (Catalog # MAB11627) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for T‑bet/TBX21 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

• Reactivity: Mu
• Applications: WB, IHC
• Clone: 1087415
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

T-bet/TBX21 Antibody (1087415) [Unconjugated] Summary

• Immunogen: Synthetic Peptide
  Accession # Q9JKD8
• Specificity: Detects a synthetic peptide specific for mouse T-bet around amino acid 20 in Direct ELISA.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3-25 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Reconstitution Instructions: Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for T-bet/TBX21 Antibody (1087415) [Unconjugated]

• Tbet
• T-bet
• TBETT-PET
• TBLYM
• TBLYMT-cell-specific T-box transcription factor T-bet
• T-box 21
• T-box expressed in T cells
• T-box protein 21
• T-box transcription factor TBX21
• TBX21
• T-PET
• Transcription factor TBLYM

Background

T-box transcription factor TBX21, also known as T-bet, is a 58kDa T-box gene which encodes transcription factors involved in the regulation of developmental processes. TBX21 is a Th1 cell-specific transcription that controls the expression of interferon-gamma. TBX21 also has an important role in many cells of the adaptive and innate immune system and is also expressed in dendritic cells, natural killer cells, natural killer T cells and innate lymphoid cells. T-bet contributes to the maintenance of mucosal homeostasis and mucosal immune response. Mice lacking adaptive immune cells and T-bet developed disease similar to human ulcerative colitis.  

1. Lazarevic V, Glimcher LH, Lord GM. T-bet: a bridge between innate and adaptive immunity. Nat Rev Immunol. 2013 Nov;13(11):777-89. doi: 10.1038/nri3536. Epub 2013 Oct 11. PMID: 24113868; PMCID: PMC6290922. 
2. Mohamed R, Lord GM. T-bet as a key regulator of mucosal immunity. Immunology. 2016 Apr;147(4):367-76. doi: 10.1111/imm.12575. Epub 2016 Feb 23. PMID: 26726991; PMCID: PMC4799884.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for T-bet/TBX21 Antibody (MAB11627). (Showing 1 - 1 of 1 FAQ).

1. Do you make a Tbx21 ab for FACS for porcine?
   • We have not tested our Tbx21 antibodies for porcine, however you might like to test NBP1-43298 as part of our Innovators Reward Program. This is gained from testing a product on a species or application that we have not yet tested. I have carried out a blast to check the % homology between the pig protein and human protein, this is 94% and our lab recommends use if the % homology is 85% or above.

Bioinformatics

• Uniprot:
  • Q9JKD8(Rabbit)