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Syndecan-4 Antibody (008)

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Immunocytochemistry/Immunofluorescence: Syndecan-4 Antibody (008) [NBP2-90699] - Immunofluorescence staining of mSDC4 in mouse spleen cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-mouse mSDC4 monoclonal antibody (dilution ratio 1:60) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to .
Immunocytochemistry/ Immunofluorescence: Syndecan-4 Antibody (008) [NBP2-90699] - Immunofluorescence staining of mSDC4 in mouse spleen cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA, ICC/IF
Clone
008
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-90699AF350
Alexa Fluor 405
NBP2-90699AF405
Alexa Fluor 488
NBP2-90699AF488
Alexa Fluor 532
NBP2-90699AF532
Alexa Fluor 594
NBP2-90699AF594
Alexa Fluor 647
NBP2-90699AF647
Alexa Fluor 700
NBP2-90699AF700
Alexa Fluor 750
NBP2-90699AF750
Biotin
NBP2-90699B
DyLight 350
NBP2-90699UV
DyLight 405
NBP2-90699V
DyLight 488
NBP2-90699G
DyLight 550
NBP2-90699R
DyLight 594
NBP2-90699DL594
DyLight 650
NBP2-90699C
DyLight 680
NBP2-90699FR
DyLight 755
NBP2-90699IR
FITC
NBP2-90699F
HRP
NBP2-90699H
Janelia Fluor 549
NBP2-90699JF549
Janelia Fluor 646
NBP2-90699JF646
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Syndecan-4 Antibody (008) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse Syndecan-4 (O35988; Met1-Val146).
Specificity
No cross-reactivity in ELISA with:

Mouse SDC1
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
SDC4
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA 1:5000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:20-1:100

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.2 um filtered solution in PBS
Preservative
No Preservative
Purity
Protein A purified

Notes

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Alternate Names for Syndecan-4 Antibody (008)

  • Amphiglycan
  • MGC22217
  • Ryudocan core protein
  • Ryudocan
  • SDC4
  • SYND4ryudocan amphiglycan
  • syndecan 4 (amphiglycan, ryudocan)
  • syndecan 4
  • syndecan proteoglycan 4
  • Syndecan4
  • Syndecan-4

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Syndecan‑1/CD138 was detected in immersion fixed paraffin-embedded sections of human jejunum using Human Syndecan‑1/CD138 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2780) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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basic/FGF2/bFGF [Unco
233-FB/CF
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NBP1-33197
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Endorepellin/Perlecan/ ...
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NBP2-23603
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[Unconjugated]
210-TA
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Bioinformatics

Gene Symbol SDC4