Synaptoporin Antibody - Azide Free

Western Blot: Synaptoporin Antibody [NB300-738] - Rat hippocampal lysate showing specific labeling of the ~38k Synaptoporin protein.

• Reactivity: Mu, Rt, Mu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide Free

Synaptoporin Antibody - Azide Free Summary

• Description: Novus Biologicals Rabbit Synaptoporin Antibody - Azide Free (NB300-738) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic peptide corresponding to amino acid residues from the C-terminal region conjugated to KLH. Accession # P22831
• Localization: Integral membrane protein; Synaptic vesicles; Cytoplasmic vesicle; secretory vesicle; synaptic vesicle membrane.
• Specificity: Specific for endogenous levels of the ~38 kDa Synaptoporin protein.
• Predicted Species: Mouse (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SYNPR
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:1000
• Theoretical MW: 38 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

It is anticipated that the antibody will work in mouse based on the fact that this species has 100% homology with the amino acid sequence used as antigen.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for Synaptoporin Antibody - Azide Free

• DKFZp686G0883
• MGC26651
• SPO
• synaptoporin

Background

Synaptophysin 2, also known as Synaptoporin, is a component of the synaptic vesicle membrane and thought to play an important role in synaptic vesicle trafficking (Dai et al., 2003). It is highly homologous to Synaptophysin 1, yet differs in expression. Whereas Synaptophysin 1 is ubiquitiously expressed, Synaptophysin 2 is concentrated in the mossy fiber synapses of the hippocampus (Grabs et al., 1994; Singec et al., 2002).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SYNPR
• Entrez:
  • 72003(Mouse)
  • 66030(Rat)
• Uniprot:
  • P22831(Rat)