Synaptopodin Antibody (G1D4)

Product Discontinued

Synaptopodin Antibody (G1D4) Summary

• Immunogen: Isolated rat kidney glomeruli
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SYNPO
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:5-1:10
  • Immunohistochemistry-Paraffin 1:5-1:10
  • Western Blot 0.1-0.5 ug/ml
• Application Notes: This antibody is useful for Immunohistochemistry on frozen sections and paraffin embedded tissue after microwave treatment: 1:5-1:10, 1 h at RT. Western blotting (the antibody also reacts with a 44 kD degradation fragment of synaptopodin). Immunofluorescence.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4) and 0.5% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Reconstitute with 1 ml distilled water to desired concetration.

Alternate Names for Synaptopodin Antibody (G1D4)

• KIAA1029
• Synaptopodin
• SYNPO

Background

Synaptopodin, a prolin-rich actin-binding protein with 2 binding sites for actin, represents a new class of actin-binding proteins which has first been localized in podocytes and a subset of telencephalic postsynaptic densities. In human tissue synaptopodin has a molecular weight of 73.7 kD and pI of 9.38 (calculated from sequence data); in mouse the corresponding data are 74 kD, pI 9.27. In SDS-PAGE the antigen appears as 100 kD polypeptide in brain and 110 kD polypeptide in kidney (the difference might be attributed to posttranslational modifications).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Synaptopodin Antibody (NBP2-33357)(1)

Publication using NBP2-33357

• Finne K, Vethe H, Skogstrand T et al. Proteomic analysis of formalin-fixed paraffin-embedded glomeruli suggests depletion of glomerular filtration barrier proteins in two-kidney one-clip hypertensive rats. Nephrol. Dial. Transplant. 2014-08-16 [PMID: 25129444]

Bioinformatics

• Gene Symbol: SYNPO