SUR1 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: SUR1 Antibody [NBP2-34077] - Staining of human cell line U-2 OS shows localization to nucleoli, cytosol & the Golgi apparatus. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: SUR1 Antibody [NBP2-34077] - Staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.

Immunohistochemistry: SUR1 Antibody [NBP2-34077] - IHC using NBP2-34077 at a dilution of 1:50 on FFPE human placenta. Primary antibody was incubated overnight at 4 C, then secondary HRP conjugate applied for 1 hour RT and visualized with DAB. HIER performed with citrate buffer pH 6.0 at 95 C for 15 minutes. Antibody incubation with 10x immunogenic peptide shows no staining. Image submitted by a verified customer review.

Immunohistochemistry-Paraffin: SUR1 Antibody [NBP2-34077] - Staining of human pancreas shows weak cytoplasmic positivity in islets of Langerhans.

Immunohistochemistry-Paraffin: SUR1 Antibody [NBP2-34077] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.

Immunohistochemistry-Paraffin: SUR1 Antibody [NBP2-34077] - Staining of human liver shows no positivity in hepatocytes as expected.

• Reactivity: Hu, Mu, Rt
• Applications: ICC/IF, IHC, Mycoplasma
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SUR1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit SUR1 Antibody - BSA Free (NBP2-34077) is a polyclonal antibody validated for use in IHC and ICC/IF. Anti-SUR1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: DHLGKENDVFQPKTQFLGVYFVSSQ
• Predicted Species: Rat (96%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ABCC8
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Knockdown Validated
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. Use in Knockdown Validated reported in scientific literature (PMID: 31067750). SUR1 antibody validated for IHC from a verified customer review.
• Control Peptide:

  SUR1 Recombinant Protein Antigen (NBP2-34077PEP)

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 31067750).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for SUR1 Antibody - BSA Free

• ABC36
• ABCC8
• ATP-binding cassette, sub-family C (CFTR/MRP), member 8
• HHF1Sulfonylurea receptor 1
• HI
• HRINS
• PHHIHRINS
• sulfonylurea receptor (hyperinsulinemia)
• SUR
• SUR1
• SUR1MRP8
• SURATP-binding cassette transporter sub-family C member 8
• TNDM2ATP-binding cassette sub-family C member 8

Background

SUR1 is encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. This protein functions as a modulator of ATP-sensitive potassium channels and insulin release. Mutations and deficiencies in this protein have been observed in patients with hyperinsulinemic hypoglycemia of infancy, an autosomal recessive disorder of unregulated and high insulin secretion. Mutations have also been associated with non-insulin-dependent diabetes mellitus type II, an autosomal dominant disease of defective insulin secretion. Alternative splicing of this gene has been observed; however, the transcript variants have not been fully described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SUR1 Antibody (NBP2-34077)(1)

Publication using NBP2-34077

• Krawchuk M Modulation of SUR1 KATP Channel Subunit Activity in the Peripheral Nervous System Reduces Mechanical Hyperalgesia after Nerve Injury in Mice Int J Mol Sci 2019-05-07 [PMID: 31067750] (KD, ICC/IF, Mouse)

Review for SUR1 Antibody (NBP2-34077) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Peter Caradonna IHC Human 04/03/2017

Summary

• Application: Immunohistochemistry
• Sample Tested: Placental tissue
• Species: Human

Comments

• Comments: IHC using NBP2-34077 at a dilution of 1:50 on FFPE human placenta. Primary antibody was incubated overnight at 4 C, then secondary HRP conjugate applied for 1 hour RT and visualized with DAB. HIER performed with citrate buffer pH 6.0 at 95 C for 15 minutes. Antibody incubation with 10x immunogenic peptide shows no staining.

Bioinformatics

• Gene Symbol: ABCC8
• Uniprot:
  • Q09428(Human)