Sulfatase Modifying Factor 1/SUMF1 Antibody [Unconjugated]

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Jurkat human acute T cell leukemia cell line, K562 human chronic myelogenous leukemia cell line, MCF-7 human breast cancer cell line, and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Sulfatase Modifying Factor 1/SUMF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3680) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for Sulfatase Modifying Factor 1/SUMF1 at approximately 40 and 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Sulfatase Modifying Factor 1/SUMF1 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human SUMF1 isoform 1
  Ser34-Asp374 with a Ser63Asn substitution
  Accession # Q8NBK3
• Specificity: Detects human SUMF1 in direct ELISAs and Western blots. In these formats, approximately 45% cross-reactivity with recombinant mouse SUMF1 is observed and less than 1% cross-reactivity with recombinant human SUMF2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SUMF1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Sulfatase Modifying Factor 1/SUMF1 Antibody [Unconjugated]

• AAPA3037
• EC 1.8.99.-
• FGE1
• FGEC-alpha-formylglycine-generating enzyme 1
• FGly-generating enzyme
• MGC131853
• MGC150436
• sulfatase modifying factor 1
• sulfatase-modifying factor 1
• SUMF1
• UNQ3037

Background

Human SUMF-1 (also FGE) is a 42 kDa (341 aa), Ca⁺⁺-binding member of the sulfatase-modifying factor family. It is a soluble ER lumenal glycoprotein that converts inactive sulfatases into an active form by transforming a catalytic site cysteine into a formylglycine residue. In the ER, SUMF-1 can exist as either a monomer, or a disulfide-linked homodimer or a heterodimer with SUMF-2. Three splice isoforms are known. Mature human SUMF-1 shares 90% aa sequence identity with mouse SUMF-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Sulfatase Modifying Factor 1/SUMF1 Antibody (AF3680)(1)

Publication using AF3680

• L Schlotawa, K Tyka, M Kettwig, RC Ahrens-Nic, M Baud, T Berulava, N Brunetti-P, A Gagne, ZM Herbst, JA Maguire, J Monfregula, T Pena, K Radhakrish, S Schröder, EA Waxman, A Ballabio, T Dierks, A Fischer, DL French, MH Gelb, J Gärtner Drug screening identifies tazarotene and bexarotene as therapeutic agents in multiple sulfatase deficiency Embo Molecular Medicine, 2023-02-15;0(0):e14837. 2023-02-15 [PMID: 36789546] (Western Blot, Human)

Bioinformatics

• Gene Symbol: SUMF1
• Uniprot:
  • Q8NBK3()