Sulfatase Modifying Factor 1/SUMF1 Antibody [Alexa Fluor® 647]

• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 647

Sulfatase Modifying Factor 1/SUMF1 Antibody [Alexa Fluor® 647] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human SUMF1 isoform 1
  Ser34-Asp374 with a Ser63Asn substitution
  Accession # Q8NBK3
• Specificity: Detects human SUMF1 in direct ELISAs and Western blots. In these formats, approximately 45% cross-reactivity with recombinant mouse SUMF1 is observed and less than 1% cross-reactivity with recombinant human SUMF2 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Human SUMF-1 (also FGE) is a 42 kDa (341 aa), Ca⁺⁺-binding member of the sulfatase-modifying factor family. It is a soluble ER lumenal glycoprotein that converts inactive sulfatases into an active form by transforming a catalytic site cysteine into a formylglycine residue. In the ER, SUMF-1 can exist as either a monomer, or a disulfide-linked homodimer or a heterodimer with SUMF-2. Three splice isoforms are known. Mature human SUMF-1 shares 90% aa sequence identity with mouse SUMF-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.