STING/TMEM173 Antibody (723505) [Alexa Fluor® 488]

Human peripheral blood mononuclear cells (PBMC) monocytes were stained with Mouse Anti-Human STING/TMEM173 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC7169G, filled histogram) or isotype control antibody (Catalog # IC0041G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

THP-1 human acute monocytic leukemia cell line was stained with Mouse Anti-Human STING/TMEM173 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC7169G, filled histogram) or isotype control antibody (Catalog # IC0041G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human STING/TMEM173 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC7169G, filled histogram) or isotype control antibody (Catalog # IC0041G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

• Reactivity: Hu
• Applications: Flow
• Clone: 723505
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

STING/TMEM173 Antibody (723505) [Alexa Fluor® 488] Summary

• Immunogen: E. coli-derived recombinant human STING/TMEM173
  Ala215-Ser379
  Accession # Q86WV6
• Specificity: Detects human STING/TMEM173 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: STING1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Intracellular Staining by Flow Cytometry 5 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for STING/TMEM173 Antibody (723505) [Alexa Fluor® 488]

• endoplasmic reticulum IFN stimulator
• Endoplasmic reticulum interferon stimulator
• ERIS
• FLJ38577
• hMITA
• hSTING
• Mediator of IRF3 activation
• MITA
• mitochondrial mediator of IRF3 activation
• MPYS
• NET23
• N-terminal methionine-proline-tyrosine-serine plasma membrane tetraspanner
• SAVI
• Stimulator of interferon genes protein
• stimulator of interferon protein
• STING
• STING-beta
• TMEM173
• transmembrane protein 173

Background

STING (Stimulator of Interferon Genes), also called ERIS, MPYS, or MITA and designated TMEM173, is a 40-42 kDa 4-transmembrane protein that mediates both antiviral and MHC-II antigen recognition responses. STING is found predominantly in the endoplasmic reticulum. It acts as an adaptor protein for intracellular viral detection molecules, participating in the induction of type I interferon. It also may play a role in the initiation of apoptosis following MHC-II engagement. Cells known to express STING include B cells, dendritic cells, macrophages, and monocytes. Human STING is 379 amino acids (aa) in length. It contains an N-terminal cytoplasmic region (aa 1-20), four transmembrane segments (aa 21-173), and a C-terminal cytoplasmic domain (aa 174-379). Ubiquitination occurs at Lys150, and phosphorylation occurs at Ser358. STING forms 80 kDa homodimers. There are two potential splice forms, one that shows a 25 aa substitution for aa 1-173, and another that possesses an alternative start site at Met215, coupled to a premature truncation following Arg334. Over aa 215-379, human and mouse STING share 76% aa sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for STING/TMEM173 Antibody (IC7169G)(1)

Publication using IC7169G

• D Kabelitz, M Zarobkiewi, M Heib, R Serrano, M Kunz, G Chitadze, D Adam, C Peters Signal strength of STING activation determines cytokine plasticity and cell death in human monocytes Scientific Reports, 2022-10-24;12(1):17827. 2022-10-24 [PMID: 36280676] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: STING1
• Uniprot:
  • Q86WV6(Human)