STAT5A Antibody (OTI9F7)

Images

 
Western Blot: STAT5A Antibody (OTI9F7) [NBP2-00622] - Analysis of extracts (35ug) from 9 different cell lines by using anti-STAT5A monoclonal antibody.
Immunohistochemistry-Paraffin: STAT5A Antibody (OTI9F7) [NBP2-00622] - Staining of paraffin-embedded Human prostate tissue using anti-STAT5A mouse monoclonal antibody.
Flow Cytometry: STAT5A Antibody (OTI9F7) [NBP2-00622] - Analysis of Jurkat cells, using anti-STAT5A antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Western Blot: STAT5A Antibody (OTI9F7) [NBP2-00622] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY STAT5A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell ...read more
Immunohistochemistry-Paraffin: STAT5A Antibody (OTI9F7) [NBP2-00622] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-STAT5A mouse monoclonal antibody.
Immunohistochemistry-Paraffin: STAT5A Antibody (OTI9F7) [NBP2-00622] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-STAT5A mouse monoclonal antibody.
Immunohistochemistry-Paraffin: STAT5A Antibody (OTI9F7) [NBP2-00622] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-STAT5A mouse monoclonal antibody.
Immunohistochemistry-Paraffin: STAT5A Antibody (OTI9F7) [NBP2-00622] - Staining of paraffin-embedded Human Kidney tissue using anti-STAT5A mouse monoclonal antibody.
Immunohistochemistry-Paraffin: STAT5A Antibody (OTI9F7) [NBP2-00622] - Staining of paraffin-embedded Human lymph node tissue using anti-STAT5A mouse monoclonal antibody.
Immunohistochemistry-Paraffin: STAT5A Antibody (OTI9F7) [NBP2-00622] - Staining of paraffin-embedded Human lymphoma tissue using anti-STAT5A mouse monoclonal antibody.
Flow Cytometry: STAT5A Antibody (OTI9F7) [NBP2-00622] - Analysis of Hela cells, using anti-STAT5A antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, Rt, Ca, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
OTI9F7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.73 mg/ml

Order Details

STAT5A Antibody (OTI9F7) Summary

Immunogen
Full length human recombinant protein of human STAT5A (NP_003143) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
STAT5A
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:150
Theoretical MW
90.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
STAT5A Knockout HeLa Cell Lysate
Reviewed Applications
Read 2 Reviews rated 4
using
NBP2-00622 in the following applications:

Publications
Read Publications using
NBP2-00622 in the following applications:

  • IHC
    1 publication

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.73 mg/ml
Purity
Affinity purified

Alternate Names for STAT5A Antibody (OTI9F7)

  • MGF
  • signal transducer and activator of transcription 5A
  • STAT5
  • STAT5a

Background

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for STAT5A Antibody (NBP2-00622)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC.


Filter By Application
IHC
(1)
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Filter By Species
Human
(1)
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Reviews for STAT5A Antibody (NBP2-00622) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using NBP2-00622:
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Filter by Species
Human
(2)
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Images Ratings Applications Species Date Details
 STAT5A NBP2-00622
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5
reviewed by:
Rognvald Blance
Human 03/22/2017
View

Summary

Sample TestedK562 human chronic myelogenous leukemia cell line
SpeciesHuman
LotA01
 STAT5A NBP2-00622
Enlarge
3
reviewed by:
Rognvald Blance
Human 02/06/2017
View

Summary

Sample TestedK562 human chronic myelogenous leukemia cell line
SpeciesHuman
LotA01

Comments

CommentsCells used = K562 or K562 + imatinib - lysed in Protein Simple bicine/CHAPS buffer (040-764) loaded at 100 ng/µL; Gradient = 5 – 8 nested, ladder# 3; Primary antibody at 1:50; Secondary antibody = Protein Simple biotinylated anti-mouse (041-127) with Protein Simple streptavidin-HRP (041-106) both at 1:100.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for STAT5A Antibody (NBP2-00622) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-74392AF350
Alexa Fluor 405 NBP2-74392AF405
Alexa Fluor 488 NBP2-74392AF488
Alexa Fluor 532 NBP2-74392AF532
Alexa Fluor 594 NBP2-74392AF594
Alexa Fluor 647 NBP2-74392AF647
Alexa Fluor 700 NBP2-74392AF700
Alexa Fluor 750 NBP2-74392AF750
Allophycocyanin NBP2-74392APC
Biotin NBP2-74392B
DyLight 350 NBP2-74392UV
DyLight 405 NBP2-74392V
DyLight 488 NBP2-74392G
DyLight 550 NBP2-74392R
DyLight 594 NBP2-74392DL594
DyLight 650 NBP2-74392C
DyLight 680 NBP2-74392FR
DyLight 755 NBP2-74392IR
FITC NBP2-74392F
HRP NBP2-74392H
Janelia Fluor 549 NBP2-74392JF549
Janelia Fluor 646 NBP2-74392JF646
PE NBP2-74392PE
PerCP NBP2-74392PCP

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Blogs on STAT5A.

Signal Transducer and Activator of Transcription STAT6: More than a Player in Allergic Inflammation
By Jamshed Arslan, Pharm. D., PhD. What is STAT6?The cellular pathway comprising tyrosine kinase Janus Kinase (JAK) and the transcription factor STAT connect extracellular signals from various cytokines, hormones an...  Read full blog post.

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Recent Reviews

4
5
1
4
0
3
1
2
0
1
0

Rognvald Blance
03/22/2017
Application:
Species: Human

Rognvald Blance
02/06/2017
Application:
Species: Human

Bioinformatics

Gene Symbol STAT5A
Entrez