STAT5A Antibody (OTI9F7)

Western Blot: STAT5A Antibody (OTI9F7) [NBP2-00622] - Analysis of extracts (35ug) from 9 different cell lines by using anti-STAT5A monoclonal antibody.

Immunohistochemistry-Paraffin: STAT5A Antibody (OTI9F7) [NBP2-00622] - Staining of paraffin-embedded Human prostate tissue using anti-STAT5A mouse monoclonal antibody.

Flow Cytometry: STAT5A Antibody (OTI9F7) [NBP2-00622] - Analysis of Jurkat cells, using anti-STAT5A antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Western Blot: STAT5A Antibody (OTI9F7) [NBP2-00622] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY STAT5A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-STAT5A.

Immunohistochemistry-Paraffin: STAT5A Antibody (OTI9F7) [NBP2-00622] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-STAT5A mouse monoclonal antibody.

Immunohistochemistry-Paraffin: STAT5A Antibody (OTI9F7) [NBP2-00622] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-STAT5A mouse monoclonal antibody.

Immunohistochemistry-Paraffin: STAT5A Antibody (OTI9F7) [NBP2-00622] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-STAT5A mouse monoclonal antibody.

Immunohistochemistry-Paraffin: STAT5A Antibody (OTI9F7) [NBP2-00622] - Staining of paraffin-embedded Human Kidney tissue using anti-STAT5A mouse monoclonal antibody.

Immunohistochemistry-Paraffin: STAT5A Antibody (OTI9F7) [NBP2-00622] - Staining of paraffin-embedded Human lymph node tissue using anti-STAT5A mouse monoclonal antibody.

Immunohistochemistry-Paraffin: STAT5A Antibody (OTI9F7) [NBP2-00622] - Staining of paraffin-embedded Human lymphoma tissue using anti-STAT5A mouse monoclonal antibody.

Flow Cytometry: STAT5A Antibody (OTI9F7) [NBP2-00622] - Analysis of Hela cells, using anti-STAT5A antibody, (Red), compared to a nonspecific negative control antibody (Blue).

• Reactivity: Hu, Mu, Rt, Ca, Pm, Pm
• Applications: WB, Flow, ICC/IF, IHC
• Clone: OTI9F7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.73 mg/ml

STAT5A Antibody (OTI9F7) Summary

• Immunogen: Full length human recombinant protein of human STAT5A (NP_003143) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: STAT5A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
• Theoretical MW: 90.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.73 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for STAT5A Antibody (OTI9F7)

• MGF
• signal transducer and activator of transcription 5A
• STAT5
• STAT5a

Background

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for STAT5A Antibody (NBP2-00622)(2)

Publications using NBP2-00622

• Olszewska B, Zawrocki A, Lakomy J et al. Mapping signal transducer and activator of transcription (STAT) activity in different stages of mycosis fungoides and Sezary syndrome Int. J. Dermatol. 2020-07-08 [PMID: 32643174]
• Slawinska M, Lakomy J, Biernat W et al. STAT3, STAT5A, STAT5B, STAT6 proteins are overexpressed in human basal cell carcinoma Clin. Exp. Dermatol. 2019-07-19 [PMID: 31323143] (IF/IHC, Human)

Reviews for STAT5A Antibody (NBP2-00622) (2)

Average Rating: 4

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Human.

reviewed by: Rognvald Blance Simple Western Human 03/22/2017

Summary

• Application: Simple Western
• Sample Tested: K562 human chronic myelogenous leukemia cell line
• Species: Human
• Lot: A01

reviewed by: Rognvald Blance Simple Western Human 02/06/2017

Summary

• Application: Simple Western
• Sample Tested: K562 human chronic myelogenous leukemia cell line
• Species: Human
• Lot: A01

Comments

• Comments: Cells used = K562 or K562 + imatinib - lysed in Protein Simple bicine/CHAPS buffer (040-764) loaded at 100 ng/µL; Gradient = 5 – 8 nested, ladder# 3; Primary antibody at 1:50; Secondary antibody = Protein Simple biotinylated anti-mouse (041-127) with Protein Simple streptavidin-HRP (041-106) both at 1:100.

Bioinformatics

• Gene Symbol: STAT5A
• Entrez:
  • 6776(Human)