StAR Antibody (HL1360) - Azide and BSA Free

Western Blot: StAR Antibody (HL1360) - Azide and BSA Free [NBP3-25696] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with StAR antibody [HL1360] (NBP3-25696) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Western Blot: StAR Antibody (HL1360) - Azide and BSA Free [NBP3-25696] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with StAR antibody [HL1360] (NBP3-25696) diluted at 1:200000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: StAR Antibody (HL1360) - Azide and BSA Free [NBP3-25696] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with StAR antibody [HL1360] (NBP3-25696) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Paraffin: StAR Antibody (HL1360) - Azide and BSA Free [NBP3-25696] - StAR antibody [HL1360] detects StAR protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. StAR stained by StAR antibody [HL1360] (NBP3-25696) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: StAR Antibody (HL1360) - Azide and BSA Free [NBP3-25696] - StAR antibody [HL1360] detects StAR protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded dog kidney. StAR stained by StAR antibody [HL1360] (NBP3-25696) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunocytochemistry/Immunofluorescence: StAR Antibody (HL1360) - Azide and BSA Free [NBP3-25696] - StAR antibody [HL1360] detects StAR protein by immunofluorescent analysis. Sample: K562 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: StAR stained by StAR antibody [HL1360] (NBP3-25696) diluted at 1:500. Blue: Fluoroshield with DAPI .

Immunohistochemistry-Paraffin: StAR Antibody (HL1360) - Azide and BSA Free [NBP3-25696] - StAR antibody [HL1360] detects StAR protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded cat kidney. StAR stained by StAR antibody [HL1360] (NBP3-25696) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: StAR Antibody (HL1360) - Azide and BSA Free [NBP3-25696] - StAR antibody [HL1360] detects StAR protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded mouse adrenal gland. StAR stained by StAR antibody [HL1360] (NBP3-25696) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

• Reactivity: Hu, Mu, Ca, Fe, Po, RM
• Applications: WB, ICC/IF, IHC
• Clone: HL1360
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

StAR Antibody (HL1360) - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit StAR Antibody (HL1360) - Azide and BSA Free (NBP3-25696) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein encompassing a sequence within the center region of human StAR. The exact sequence is proprietary.
• Source: HEK293
• Predicted Species: Porcine (90%), Rhesus Macaque (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: STAR
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (89%), Sheep (86%), Bovine (87%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for StAR Antibody (HL1360) - Azide and BSA Free

• cholesterol trafficker
• mitochondrial steroid acute regulatory protein
• StAR
• StARD1
• STARD1StAR-related lipid transfer (START) domain containing 1
• START domain containing 1
• START domain-containing protein 1
• steroid acute regulatory protein
• steroidogenic acute regulator
• steroidogenic acute regulatory protein
• steroidogenic acute regulatory protein, mitochondrial

Background

StAR is encoded by this gene plays a key role in the acute regulation of steroid hormone synthesis by enhancing the conversion of cholesterol into pregnenolone. This protein permits the cleavage of cholesterol into pregnenolone by mediating the transport of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane. Mutations in this gene are a cause of congenital lipoid adrenal hyperplasia (CLAH), also called lipoid CAH. A pseudogene of this gene is located on chromosome 13.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: STAR