ST3 beta-Gal alpha-2,3-Sialyltransferase 1/ST3GAL1/SIAT4A Antibody - BSA Free

Western Blot: ST3 beta-Gal alpha-2,3-Sialyltransferase 1/ST3GAL1/SIAT4A Antibody [NBP1-87045] - Analysis in human plasma.

Immunohistochemistry-Paraffin: ST3 beta-Gal alpha-2,3-Sialyltransferase 1/ST3GAL1/SIAT4A Antibody [NBP1-87045] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.

Immunohistochemistry-Paraffin: ST3 beta-Gal alpha-2,3-Sialyltransferase 1/ST3GAL1/SIAT4A Antibody [NBP1-87045] -Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.

Immunohistochemistry-Paraffin: ST3 beta-Gal alpha-2,3-Sialyltransferase 1/ST3GAL1/SIAT4A Antibody [NBP1-87045] -Staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.

Immunohistochemistry-Paraffin: ST3 beta-Gal alpha-2,3-Sialyltransferase 1/ST3GAL1/SIAT4A Antibody [NBP1-87045] -Staining of human skin shows very weak cytoplasmic positivity in squamous epithelial cells as expected.

Analysis in human plasma.

Staining of human skin shows very weak cytoplasmic positivity in squamous epithelial cells as expected.

Staining of human liver shows strong cytoplasmic positivity in hepatocytes.

Staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.

Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ST3 beta-Gal alpha-2,3-Sialyltransferase 1/ST3GAL1/SIAT4A Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: MVLELSENLKRLIKHRPCTCTHCIGQRKLSAWFDERFNQTMQPLLTAQNALLEDDTYRWWLRLQREKKPNNLNDTIKELFRVVPGNVDPMLEKRSVGCRRCAVVGNSGNLRESSYGPEIDSHDFVLRMNKAPTAGFEA
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ST3GAL1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  ST3 beta-Gal alpha-2,3-Sialyltransferase 1/ST3GAL1/SIAT4A Protein (NBP1-87045PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (81%), Rat (80%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for ST3 beta-Gal alpha-2,3-Sialyltransferase 1/ST3GAL1/SIAT4A Antibody - BSA Free

• Alpha 2,3-ST 1
• CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 1
• DKFZp666E036
• DKFZp779K2051
• EC 2.4.99
• EC 2.4.99.4
• Gal-beta-1,3-GalNAc-alpha-2,3-sialyltransferase
• Gal-NAc6S
• sialyltransferase 4A (beta-galactosidase alpha-2,3-sialytransferase)
• sialyltransferase 4A (beta-galactoside alpha-2,3-sialyltransferase)
• sialyltransferase 4A (beta-galactoside alpha-2,3-sialytransferase)
• Sialyltransferase 4A
• SIAT4
• SIAT4A
• SIAT4-A
• SIATFL
• SIATFLFLJ36548
• ST3 beta-galactoside alpha-2,3-sialyltransferase 1
• ST3Gal I
• ST3GAL1
• ST3GalA
• ST3GalA.1MGC9183
• ST3GalI
• ST3GalIA
• ST3GalIA1,Beta-galactoside alpha-2,3-sialyltransferase 1
• ST3O
• ST3OST3GalA

Background

SIAT4A is encoded by this gene is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The encoded protein is normally found in the Golgi but can be proteolytically processed to a soluble form. Correct glycosylation of the encoded protein may be critical to its sialyltransferase activity. This protein, which is a member of glycosyltransferase family 29, can use the same acceptor substrates as does sialyltransferase 4B. Two transcript variants encoding the same protein have been found for this gene. Other transcript variants may exist, but have not been fully characterized yet. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ST3GAL1